【作者】 王任钦；

【导师】 唐瞻贵；

【作者基本信息】 中南大学 ， 口腔临床医学， 2008， 硕士

【摘要】 目的:研究S期激酶相关蛋白Skp2与细胞周期素抑制因子p27在口腔疣状癌中的表达特点,探讨其与临床病理特点的关系,为口腔疣状癌的诊断、治疗及预后评估提供新的思路,为进一步了解口腔疣状癌与口腔鳞状细胞癌的区别和联系提供参考。方法:采用免疫组化SP法检测15例口腔疣状癌、30例口腔鳞癌和15例正常口腔粘膜标本中Skp2和p27蛋白的表达水平,SPSS12.0软件对数据进行统计学分析。结果:1.Skp2的表达Skp2在口腔疣状癌组、鳞癌组中的表达显著高于正常组（P＜0.05）,疣状癌和鳞癌组间比较无显著性差异（P＞0.05）。疣状癌组中,按年龄、性别、肿瘤部位及临床分期等分组,Skp2的表达均无显著性差异（P＞0.05）;按临床分型分组,Skp2表达有显著差异（P＜0.05）。鳞癌组中,按年龄、性别、肿瘤部位等分组,Skp2的表达均无显著性差异（P＞0.05）,按临床分期和恶性程度分组,临床分期越高、分化程度越低、有淋巴结转移者,Skp2表达越高,有显著性差异（P＜0.05）。2.p27的表达p27在口腔疣状癌组、鳞癌组、和正常组三组的阳性表达率分别为43.3%,53.3%和93.3%,三组总体比较有显著性差异（P＜0.05）。口腔疣状癌组、鳞癌组中的表达显著低于正常组（P＜0.05）,疣状癌和鳞癌组间比较无显著性差异（P＞0.05）。疣状癌组中,按年龄、性别、肿瘤部位及临床分期等分组,p27的表达均无显著性差异（P＞0.05）;按临床分型分组,浸润/囊肿型p27表达显著低于外生型;鳞癌组中,按年龄、性别、肿瘤部位、临床分期等分组,p27的表达均无显著性差异（P＞0.05）,按恶性程度分组,分化程度越低、有淋巴结转移者,p27表达越低,差异有显著性（P＜0.05）。3.Spearman等级相关分析表明,口腔疣状癌、口腔鳞癌组织中Skp2,p27表达均呈明显负相关（r₁=-0.935,P＜0.05）、（r₂=-0.639,P＜0.05）。结论:1.Skp2和p27可能参与了口腔疣状癌和口腔鳞癌的发生、发展过程,并与肿瘤的恶性程度密切相关。2.Skp2和p27可能在口腔疣状癌和口腔鳞癌的侵袭和转移过程中起起作用。3.Skp2可能是造成恶性肿瘤中p27表达下调的重要因素。更多还原

【Abstract】 Objective:To study the expression of Skp2（S-Phase kinase associated Protein 2）and cyclin-dependent kinase inhibitor p27 in the oral verrucous carcinoma（OVC）and their relationship with clinicoPathologic characters,so as to provide a new way for early diagnosis,prognosis assessment and treatment of OVC.Besides,the results were compared with those of oral squamous cell carcinoma （OSCC）to discuss their difference and relationship.Methods:The immunohistochemistry technic was used to detect the expression of Skp2 and p27 in 15 OVC tissues,30 oral squamous carcinoma（OSCC）tissues and 15 normal oral mucosa tissues.Statistical analysis was performed by SPSS 12.0 software.Results:1.The expression of Skp2 The expression rates of Skp2 in the OVCs and OSCCs were significantly higher than those in normal mucosa （P＜0.05）,but there was no difference of Skp2 expression among the OVCs and OSCCs（P＞0.05）.In the OVCs,There was no difference of Skp2 expression in sex,age of the patients,as well as the tumour site or stage（P＞0.05）.For different phenotypes of OVC,the positive expression rate samples were different（P＜0.05）,while in the OSCCs,Skp2 expression was significantly associated with stages,differentiated grades and lymph node metastasis.The samples with higher stage,poorer differentiated grade or more probability lymph node metastasis had a significantly higher expression of Skp2.2.The expression of p27 The expression rates of p27 in the OVCs（43.5%）and OSCCs（53.3%）were significantly higher than those in normal mucosa（93.3%）（P＜0.05）.But there was no difference of p27 expression among the OVCs and OSCCs（P＞0.05）.In the OVCs,there was no difference of p27 expression in sex,age of the patients,tumour site,or stage（P＞0.05）.The positive expression rate samples of different types of OVC were different（P＜0.05）,while in the OSCCs,p27 expression was significantly associated with differentiated grades and lymph node metastasis,p27 staining in poorly differentiated carcinoma or more probability lymph node metastasis is remarkably reduced.3.Spearson anlysis showed that Skp2 was inversely associated with expression of p27 in OVC.（r₁=-0.935,P<0.01）,and Skp2 was inversely associated with expression of p27 in OSCC.（r₂=-0.639,P＜0.01）Conclusions:1.The expression of Skp2 and p27 may contribute to the carcinogenesis of OVC and OSCC,and has close relationship with the malignant potential of tumors.2.Skp2 and p27 may contribute to the progression and metastasis of OVC and OSCC.3.Skp2 plays an important role in the down-regulation of p27 during oral cancer development.更多还原