【作者】 张凯；

【导师】 彭解英；

【作者基本信息】 中南大学 ， 口腔临床医学， 2008， 硕士

【摘要】 目的:通过研究整合素连接激酶(integrin-linked kinase,ILK)在口腔粘膜下纤维化(oral submucous fibrosis,OSF)病变组织中的表达与分布,探讨ILK与OSF病变发生发展的关系。方法:1、采用免疫组织化学染色的方法,观察ILK在15例正常口腔粘膜组织和OSF早、中、晚期各25例病变组织中的表达与分布,并采用半定量方法检测正常口腔粘膜组和OSF各组中ILK的表达水平。2、采用逆转录聚合酶链反应研究ILK mRNA在5例正常口腔粘膜组织和8例OSF早期病变组织、15例OSF中期病变组织、6例OSF晚期病变组织中的表达水平。结果:1、正常口腔粘膜上皮组织中,ILK在个别基底层和棘层细胞的胞浆和胞核中表达;随着OSF病变程度的加重,表达ILK的细胞逐渐增多,着色程度逐渐加深,到了晚期组,胞浆和胞核呈深红染色的细胞几乎遍布上皮全层。2、ILK蛋白在OSF各组中的表达均较正常口腔粘膜高(p＜0.05);ILK蛋白在OSF早期组中的表达明显低于OSF中、晚期组(p＜0.05);ILK蛋白在OSF中期组中的表达低于OSF晚期组,但两者比较无统计学意义(p＞0.05)。3、ILK mRNA在OSF各组中的表达均较正常口腔粘膜高(p＜0.05):ILK mRNA在OSF早期组中的表达明显低于OSF中、晚期组(p＜0.05);ILK mRNA在OSF中期组中的表达低于OSF晚期组,但两者比较无统计学意义(p＞0.05)。结论:1.在OSF病变上皮组织中,ILK蛋白均有明显的高表达,且随着病程的发展,其表达逐步增强。2.在OSF病变组织中,ILK mRNA的表达水平明显增高,且随着病程的发展,其表达逐步增强。3.过量表达的ILK能够通过多种病理生理途径促进OSF的发生与发展。更多还原

【Abstract】 Purpose: To study the expression and location of integrin-linked kinase (ILK) in oral submucous fibrosis(OSF), and to characterize the role of this kinase in the pathogenesis of OSF.Methods:1. ILK protein was analysed by immunohistochemistry in 15 cases of normal oral mucosa and 75 cases of OSF lesion tissues, including 25 cases of early, middle and late stage respectively, and semi-quantitative method was used for detecting the expression levels of ILK in normal oral mucosa group and each OSF group.2. Transcription levels of ILK mRNA were determined by reverse transcription-polymerase chain reaction (RT-PCR) within 5 cases of normal oral mucosa and 29 cases of OSF lesion tissues, including 8 cases of early stage, 15 cases of middle stage, 6 cases of late stage.Results:1. In normal oral epithelial tissue, ILK was expressed weakly in the nuclear and the cytoplasm of individual cells of the basal layer and the spinous layer. With the increasing severity of OSF, the cells expressing ILK increased gradually, the staining degree deepened gradually. In the late stage, the cells stained with dark red in the nuclear and the cytoplasm were almost around the entire layers.2. ILK expression was significantly higher in each OSF group than that in normal group (p<0.05). ILK expression in early stage was significantly lower than that in middle and late stage (p<0.05). ILK expression in middle stage was not significantly lower than that in late stage (p>0.05). 3. ILK mRNA expression was significantly higher in each OSF group than that in normal group (p<0.05). ILK mRNA expression in early stage was significantly lower than that in middle and late stage (p<0.05). ILK mRNA expression in middle stage was not significantly lower than that in late stage (p>0.05).Conclusions:1. Integrin-linked kinase is overexpressed in the epithelial layer of OSF lesion tissues. With the development of disease, the expression levels increased gradually.2. ILK mRNA is overexpressed in the lesion tissues of OSF. With the development of disease, the expression level increased gradually.3. The overexpression of ILK probably promotes the occurrence and development of OSF through a variety of pathophysiological ways.更多还原