【作者】 温春燕；

【导师】 高义军；

【作者基本信息】 中南大学 ， 口腔临床医学， 2008， 硕士

【摘要】 目的:检测抑癌基因脆性组氨酸三联体(FHIT)、p16蛋白在口腔黏膜下纤维性病变(Oral Submucous Fibrosis,OSF)及其伴发口腔癌组织中的表达与分布情况,初步探讨FHIT、p16在OSF癌变发生,发展过程中的作用。方法:采用免疫组化SP法,用鼠抗人FHIT、p16单克隆抗体检测44例OSF组织、15例OSF伴发的口腔癌组织及8例正常对照者的口腔粘膜组织中FHIT、p16蛋白的表达及分布。结果:(1)FHIT蛋白阳性表达定位于细胞质,少数可见细胞核黄染。FHIT蛋白在正常口腔粘膜组织高表达率为87.5%,OSF组织中的高表达率为70.4%,两者之间没有显著性差异(P＞0.05);FHIT蛋白在正常口腔粘膜组织与OSF伴发口腔癌组织中的高表达率(40.0%)有显著性差异(P＜0.05);FHIT蛋白在OSF组织与OSF伴发口腔癌组织中的表达有显著性差异(P＜0.05)。(2)p16蛋白在细胞核和细胞质均有表达,其在正常口腔粘膜组织高表达率为100.0%,在OSF组织中的高表达率72.7%,两者之间没有显著性差异(P＞0.05);p16蛋白在正常口腔粘膜组织与OSF伴发口腔癌组织中的高表达率(40.0%)有显著性差异(P＜0.05);p16蛋白在OSF组织与OSF伴发口腔癌组织中的表达有显著性差异(P＜0.05)。(3)p16蛋白在各组病变组织中表达的平均灰度值均分别小于FHIT蛋白在各其病变组织中表达的平均灰度值,差别有统计学意义(P＜0.05)。FHIT蛋白和p16蛋白在不同病变组织中的表达显示二者正相关,其Person相关系数为0.970,P值为0.030(双侧)。结论:(1)FHIT蛋白和p16蛋白在OSF伴发口腔癌组织中表达下调和缺失,可能是OSF癌变过程中的早期表现。(2)FHIT蛋白和p16蛋白在OSF及其伴发口腔癌组织中的表达呈显著正相关,表明两者可能有协同作用,共同参与OSF癌变的过程。更多还原

【Abstract】 Objective:To investigate the expression of FHIT and p16 protein in oral submucous fibrosis(OSF)and oral submucous fibrosis concomitant with oral squamous cell carcinoma(OSCC),and to evaluate their possible roles in carcinogenesis of OSF.Methods:Immunohistochemical staining by SP methods was utilized to detect the expression of FHIT,p16 in 44 cases of OSF,15 cases of oral submucous fibrosis concomitant with OSCC and 8 cases of normal control oral mucosa tissue.Results:(1)The positive rates of FHIT in normal tissues,OSF and OSF concomitant with OSCC are 87.5%,70.4%40.0%respectively. The differences which in expression between normal tissues group and carcinogenesis of OSF group,between OSF group and OSF concomitant with OSCC group are statistically significant (P＜0.05).(2)The positive rates of p16 in normal tissues,OSF and OSF concomitant with OSCC are 100.0%,72.7%,40.0%respectively. The differences which in expression between normal tissues group and carcinogenesis of OSF group,between OSF group and OSF concomitant with OSCC group are statistically significant (P＜0.05).(3)The expressions of FHIT are corresponding higher than the expression of p16 among the five groups,the differences are statistically significant(P＜0.05).There are positive correlation between the expression of FHIT and p16 in OSF and OSF concomitant with OSCC group(r=0.970,P＜0.05).Conclusions:(1)The loss of FHIT or p16 is likely to be an early occurrence in the carcinogenesis of OSF.(2)The positive correlation between the expression of FHIT and P16 demonstrats that they may act together promoting the carcinogenesis of OSF.更多还原