【作者】 康逢良；

【导师】 曹农；

【作者基本信息】 兰州大学 ， 外科学， 2008， 硕士

【摘要】 目的:观察过氧化物酶体增殖物激活受体(PPARγ)在急性胰腺炎(acutepancreatitis AP)中的表达及其与胰腺病理改变之间的关系,探讨PPARγ在AP机制中作用。方法:54只wistar大鼠随机分为轻症胰腺炎组(M组)、重症胰腺炎组(S组)、对照组(C组)应法检测胰腺组三组,采用胰胆管注射方法复制胰腺炎动物模型。在各时间点以光镜观察胰腺病变,用免疫组化和反转录聚合酶链式反织中PPARγ蛋白及PPARγmRNA的表达。结果:C组1、6、12小时各时间点PPARγ蛋白及PPARγmRNA的表达无统计学意义,M组、S组12小时点PPARγ蛋白及PPARγmRNA的表达高于1、6小时点,具有统计学意义(P＜0.05)。在12小时点S组PPARγ表达最高,其次为M组,表达最低的为C组,三组间具有统计学意义(P＜0.05)。胰腺的病理形态变化与PPARγ蛋白及PPARγmRNA的表达成正相关。结论:PPARγ参与急性胰腺炎的发生发展。与病理类型关系密切,其水平可作为急性胰腺炎的预后指标更多还原

【Abstract】 Objective:To observe the changes of PPARγin rats with acute pancreatitis,and to explore relationship between PPARγwith acute pancreatitis.Methods:Adult Wistar rats were randomly divided into three groups.Eight rats of each groups were killed at 1,6,12h points after model was established and pancreatic tissue was collected.RT-PCR and immunohistochemistry were used to detect the lever of PPARγmRNA and protein in 54 pancreatic tissue of rats with acute pancreatitis.The relationship between the expression and the pathological changes was analyzed statistically.Results:At 1 6 12hour the leves of PPARγand PPARγmRNA has no significantly in C group(p＜0.05).at 12 hour the leves of PPARγand PPARγmRNA in C Group and Mgroup is higher than Cgroups(p＜0.05);at 12 hour the expressions of PPARγand PPARγmRNA in Cgroups is highest,Cgroups is lowest(p＜0.05).The expression of PPARγand PPARγmRNA is positive correlated with the scores of pancreatic tissue pathology(r=0.341 0.311).Conclusion:PPARγmay play an important role in the pathogenesis or progression of acute pancreatitis,and the level of PPARγmay be a prognostic indicator in actue pancreatitis.更多还原