节点文献
高纯低聚果糖分离纯化技术的研究
Study on Separation and Purification Method of High-Purity Fructooligosaccharide
【作者】 武金良;
【导师】 卢家炯;
【作者基本信息】 广西大学 , 制糖工程, 2008, 硕士
【摘要】 低聚果糖具有促进人体肠道内双歧杆菌生长的显著作用,并有保护肝脏,提高机体免疫力等生理功能,广泛应用于食品和保健品行业。但大规模生产的低聚果糖产品为多种组分的混合物,纯度不高限制了公众对其生理功能的认同感。由于技术条件限制,国内市场紧缺高纯度的低聚糖产品,这使得纯化低聚果糖具有极其现实的意义。本课题初步研究了对低聚果糖糖浆的脱色效果方法。结果表明70℃,pH=3.8,活性炭用量为1.4%,脱色50min时活性炭对低聚果糖具有很好的脱色效果。新生态碳酸钙法用于低聚果糖脱色效果不理想。同时研究了利用膜分离法将脱色后的低聚果糖进行两级纳滤和一次反渗透,并将其分离为90%以上纯度和50%以上纯度的低聚果糖产物Ⅰ和产物Ⅱ以及葡萄糖和蔗糖的混合产物Ⅲ。产物Ⅰ虽然产率并不高,但却为后续凝胶层析分离低聚果糖单一组分提供了原料来源,同时也为Bio-gel P-2凝胶层析分离奠定了良好的分离基础。利用聚丙烯酰胺凝胶P-2,当流速为0.12mL·min-1,120×1cm I.D,进样量为0.5mL时,以高纯度低聚果糖为原料初步得到蔗果三糖、蔗果四糖以及蔗果五糖的单一组分,原料纯度越高,单一组分纯度也越高。葡聚糖凝胶LH-20在纯水作为流动相时分离低聚果糖单一组分不理想。运用KS与CS系列凝胶糖柱,以纯水为流动相,三柱串联方法能够有效分离低聚果糖,本研究为低聚糖类物质提供了新型、环保节能的分离方法。
【Abstract】 Fructooligosaccharide (FOS) exhibit a stimulating effect on the growth of the beneficial Bifidobacteria in animal intestines and protect liver, improve immunity etc, it’s currently used in the so-called health foods and feedstuff and so on.Because of the short of high-purity FOS, ti’s very usefull for purifying FOS. However, large-scale production of FOS is mixture of multiplicate components, it’s low-purity, restricts the public’s sense of identification with their physiological functions. Due to technical constraints, high-grade products of oligosaccharides is short in the domestic market, which makes FOS purification is extremely practical significance.This experiment preliminary studied on decolourization of FOS syrup. The experiments show that the decolourization effect was the best when the amount of activated carbon is 1.4 percent, decolorization temperature is 70℃, pH is 3.8 and bleaching time is 40 min. fresh lime carbonate is not effective for the decolourization of FOS.FOS is purified by NF after decolourization. It is purified by twice NF process and once RO, then we can get three products: productⅠ’s purity is above 90%, productⅡ’s purity is above 90%, productⅢis the mixture of sucrose and glucose. productⅠis not high yielded, but it is the raw material for the separation of FOS and it is useful for the separation with Bio-gel P-2.We can get 1-kestose, nystose and fructofuranosylnystose by separation with Bio-gel P-2 when the flow rate is 0.12mL·min-1,column longth is 120×1 cm I.D ,injection volumn is 0.5mL.They will have high purity when the raw material is more pure.It’s non-ideal for separation with sephadex LH-20 when the mobile phase is water.It’s effective for the separation with KS & CS series sugar column when the mobile phase is water and with three connected columns. This method is new, and it’s good for environmental protection.
【Key words】 Fructooligosaccharide; activated carbon; Nanofiltration; Separation and Purification; Gel filtration chromatography;