【作者】 高甜；

【导师】 吴树清；

【作者基本信息】 内蒙古农业大学 ， 临床兽医学， 2008， 硕士

【摘要】 本实验将实验动物分为局麻对照组、局麻补硒组、全麻对照组、全麻补硒组。局麻补硒组和全麻补硒组均在手术前一周按剂量补硒,并与其余两组分别在术前1w(T0)、术前(T1)、切皮后1h(T2)、术后6h(T3)、术后12h(T4)、术后1d(T5)、术后2d(T6)、术后3d(T7)、术后5d(T8)、术后1w(T9)、术后2w(T10)、术后3w(T11)、术后4w(T12)采颈静脉血,测定其血清甲状腺激素、促甲状腺激素、皮质醇活性,谷胱甘肽过氧化物酶、总抗氧化能力、丙二醛活性,摸索出其动态变化并作了统计分析。实验结果显示:术后绵羊体内的内分泌系统及抗氧化系统均有不同程度的变化。且机体的部分激素和抗氧化水平都伴随机体硒水平的变化而变化。术后四组实验羊皮质醇的显著变化反映了动物机体的应激程度,两补硒组相对于对照组应激程度趋于缓和,且较早的恢复术前水平;四组实验羊甲状腺激素变化趋势相似,但补硒组水平明显高于对照组,且术后四组均呈现不同程度的正常甲状腺病态综合征;在术前补充一定剂量的硒制剂(亚硒酸钠)后,动物机体的抗氧化能力显著增强,围术期内两补硒组T-AOC及GSH-Px活性均高于相应的对照组,补硒组MDA的含量低于对照组,说明机体内抗氧化系统与硒水平之间存在着内在的规律性的联系。实验证明术前补充适量的硒能够有效的提高机体的抗氧化能力,促进手术创伤早期恢复。更多还原

【Abstract】 20 sheep were divided into 4 groups.They were Local Anesthesia Controls,Local Anes thesia&Antioxygen,Pantanesthesia Controls and Pantanesthesia&Antioxygen.The Sodium Selenium were injected to the sheep of Pantanesthesia& Antioxygen and Anesthesia& Antioxygen one week before the rumen fistula operatited. The venous blood samples of the sheep were taken at one week before (T0),preanesthesia(T1),1h after incision(T2),6h after operation(T3),12h after operation (T4),1d after operation(T5),2d after operation(T6),3d after operation(T7),5d after operation (T8), 1w after operation(T9), 2w after operation(T10),3w after operation (T11),4w after operation (T12) separately. Thyroid hormones, thyroid stimulating hormone, Selenium, GSH-Px, T-AOC, MDA in blood serum were tested by the corresponding detection methods. The data were analysed by the statistical way.The results showed that the different degrees of variation were occurred on the endocrine system and antioxidant system. The changes of partial Hormone and the antioxidant level were fluctuating with the Content of the Se in organism. The significant changes after operation of COR were showed the stress status of the experimental sheep. The changes of the two antioxidative groups were more mitigate than those of the other groups.And those two were recovered earlier.The changing trend of the four in TH were almost the same, but the TH level of the antioxidant groups were higher than those of the non-antioxidant groups.All of the four groups were showed the symptoms of Euthyroid Sick Syndrome (ESS). The antioxidant capacity of the body was significantly enhanced after the Sodium Selenium injected.The activation of T-AOC and GSH-Px in antioxidant groups was higher than the corresponding Controls. And the changes of the content of MDA in organisms were inverse. It showed that there was a regular inner link between the oxidant defense system and Se concentration. It was found that proper Sodium Selenium injected before operation was effective for strengthenning the antioxidant abilitiy and promoting recovery.更多还原