【作者】 王淑敏；

【导师】 杨振才；

【作者基本信息】 河北师范大学 ， 动物学， 2008， 硕士

【摘要】 本试验以体重16.03±1.54g的红鳍东方鲀为研究对象,蛋白质作为试验因子;以白鱼粉为蛋白源,配制5种不同蛋白质梯度的等能实验饲料,分别饲喂5个组,进行为期45d的饲养试验,研究红鳍东方鲀的蛋白质需要量。试验鱼共300尾,随机分为5组,每组6个重复,每个重复10尾。试验饲料的蛋白质含量为:34.45%、37.08%、39.83%、43.25%、46.07%。试验结果如下:饲养试验结果:特定生长率(SGR)随饲料蛋白质含量的升高呈现先升高后降低的趋势;方差分析表明:不同饲料蛋白质含量对SGR影响显著(p<0.05);饲料蛋白质含量和SGR的关系为:y=-0.00588x2+0.4832x-7.485(r=0.39);计算得到SGR最高值2.24时,对应饲料蛋白质含量为41.08%。摄食量与饲料蛋白质含量呈显著线性负相关:y=-0.3667x+62.038(p<0.05),表明摄食量随饲料蛋白质含量的增加而逐渐减少。饲料干物质转化率(FCEdm)、蛋白质转化率(FCEp)和能量转化率(FCEe)均随饲料蛋白质含量的上升而先升高后降低;三者均在饲料蛋白质含量39.83%处达到最大。方差分析表明:饲料蛋白质含量对红鳍东方鲀FCEdm、FCEp、FCEe影响差异显著(p=0.003,p=0.0026,p=0.004)。蛋白质效率(PER)随蛋白质含量的上升亦先升后降;饲料蛋白质含量与PER关系为:y=-0.6393x2+50.248x-817.33(r=0.57)。鱼体营养组分:方差分析结果表明:饲料蛋白质含量对鱼体蛋白质和灰分含量影响显著(p<0.05);鱼体蛋白质含量随着饲料蛋白质含量的上升不断增加,并呈正相关:y=0.363x+69.70(r=0.58)。对鱼体水分、脂肪以及能量含量影响不显著;但脂肪含量随着饲料蛋白质含量的升高呈现下降趋势。营养物质表观消化率:蛋白质含量对干物质表观消化率(Ddm)及蛋白质表观消化率(D_p)影响显著(p<0.05);而且D_p与饲料蛋白质含量呈显著线性正相关:y=0.6281x+58.251(r=0.91);结果表明:饲料蛋白质含量在34.45%-46.07%之间时,D_p随饲料蛋白质含量的上升而增大。氮收支模式:不同饲料蛋白质含量下氮收支模式如下34.45%: 100C = 29.90G + 21.44F + 48.67U 37.08%: 100C = 28.67G + 18.57F + 52.76U 39.83%: 100C = 29.54G + 14.35F + 56.11U 43.25%: 100C = 27.98G + 14.90F + 57.12U 46.07%: 100C = 25.34G + 13.68F + 60.98U方差分析表明:饲料不同蛋白质含量对红鳍东方鲀氮收支模式影响显著(p<0.05)。消化道形态:消化道长度与蛋白质含量呈负相关:y=-2.282x+266.19(r=-0.44)试验结果表明:体重处于16g-50g生长阶段的红鳍东方鲀,其饲料的适宜蛋白质含量为41.08%。更多还原

【Abstract】 The experiment was conducted to study the optimum level of protein on Fugu rubripes about 16g after 45d’s feeding trial ,designed by five levels of protein. White fishmeal was used as the main dietary protein source to formulate isocaloric experimental diet .300 Fugu rubripes were devided five groups at random with 6 replications and there were 10 trial in each replication. Five group protein were 34.45%,37.08%,39.83%,43,25% and 46.07%. The main results were as follows:The feeding trial showed that SGR was increasing first and then reducing with the increasing of protein content of diets. The analysis of variance showed that different feeding protein had significant effect on SGR(p<0.05), the conic model of feeding protein content and SGR is y=-0.00588x2+0.4832x-7.485(r=0.39);the highest SGR was 2.44, the homologous protein content was 41.08%;the feed intake had negative correlation with protein content: y=-0.3667x+62.038(p<0.05), which showed that the taken diet quantity was reducing with the increasing protein content. FCEdm , FCEp and FCEe were all increasing first and then reducing with the increasing of protein content of feed. There had a highest point at 39.83% protein. The analysis of variance showed that protein content of feed had significant effect on FCEdm、FCEp、FCEe of Fugu rubripes(p=0.003,p=0.0026,p=0.004).PER was increasing first and later reducing with the protein content; the relation between proein content of feed and PER was: y=-0.6393x2+50.25x-817.33(r=0.57).The nutrition composition of the fish body: The analysis of variance showed that protein content of feed has significant effect on protein content and ash content of the fish body(p<0.05);the body protein content was increasing with the increasing of feed protein content, the relation between proein content of feed and the body protein was:y=0.363x+69.70(r=0.584); there had not significant effect on water, fat and energy of the body,while fat content of body was reducing with the increasing of feed protein content .The apparent digestibility coefficients of nutrition ingredients: protein content had significant difference in Ddm and Dp(p<0.05),and Dp had positive correlations with protein content of feed: y=0.6281x+58.251(r=0.91),the result showed that Dp was increasing with the increasing of protein content when protein content of feed was between 34.45%-46.07%.Dietry protein level had significant effect on the nitrogen budget.The nitrogen budgets of different protein content of feed were shown as follows: 34.45%: 100C = 29.90G + 21.44F + 48.67U 37.08%: 100C = 28.67G + 18.57F + 52.76U 39.83%: 100C = 29.54G + 14.35F + 56.11U 43.25%: 100C = 27.98G + 14.90F + 57.12U 46.07%: 100C = 25.34G + 13.68F + 60.98UThe alimentary canal had negative correlation with protein content: y=-2.282x+266.19(r=-0.44)The result showed that the optimum protein level of Fugu rubripes is 41.64% when its avoirdupois was between 16g-50g.更多还原