心复康口服液对心梗后心衰大鼠模型心功能及心肌能量代谢干预的研究

【作者】 陈琴；

【导师】 胡元会；

【作者基本信息】 北京中医药大学 ， 中医内科学， 2008， 硕士

【摘要】 目的:观察心复康口服液对心梗后心衰大鼠血流动力学、心功能及心肌能量代谢物质的影响。方法:采用结扎SD大鼠左冠状动脉前降支致心梗后心衰大鼠模型,大鼠随机分为假手术组(Sham组)、模型组(Model组)、Captopril组(Capt组)、益气活血中药组分为心复康口服液大剂量组(XFK大剂量组)和心复康口服液小剂量组(XFK小剂量组),分别于术后24h灌胃给药,以Captopril作为阳性对照药。给药8周末,观察大鼠血流动力学、心功能及心肌能量代谢物质的的变化。结果:(1)XFK对心梗后CHF大鼠血流动力学的影响与Sham组比较,Model组大鼠舒张压降低,统计学有显著性差异(P<0.05),心率及收缩压变化无统计学意义(P>0.05);与Model组比较,XFK组和Capt组大鼠心率及舒张压变化均无统计学意义,但Capt组大鼠收缩压明显降低,统计学有极显著性差异(P<0.01)。(2)XFK对心梗后CHF大鼠心功能的影响与Sham组比较,Model组大鼠左心室舒张末压升高,左心室收缩压、心输出量、左心室内压最大上升速率、左心室内压最大下降速率均显著降低,统计学有极显著性差异(P<0.01);与Model组比较,XFK组和Capt组大鼠左心室舒张末压降低,统计学有显著性差异(P<0.05),左心室收缩压、心输出量、左心室内压最大上升速率、左心室内压最大下降速率均升高,统计学有极显著性差异(P<0.01)。(3)XFK对CHF大鼠左心室非梗死心肌ATP、AMP、ADP水平的影响与Sham组比较,Model组大鼠左心室心肌ATP含量降低,ADP、AMP含量明显增高,统计学有极显著性意义(P<0.01);与Model组比较,XFK组和Capt组大鼠左心室心肌ATP水平明显升高,ADP和AMP含量明显降低,统计学有极显著性意义(P<0.01)。结论:(1)心复康口服液对心梗后心衰大鼠心率、BSP、BDP无影响,可有效降低心梗后心衰大鼠心脏LVEDP,提高心梗后心衰大鼠LVSP,改善心梗大鼠血液动力学稳态。(2)心复康口服液可有效提高心梗后心衰大鼠心脏+dp/dtmax、CO,从而改善心梗后心衰大鼠左室收缩和舒张功能。(3)心复康口服液可有效提高心梗后心衰大鼠心肌能量代谢产物ATP含量,减少AMP、ADP的含量。说明益气活血中药可有效调节心梗后心衰大鼠心肌能量代谢紊乱,改善心梗后心衰大鼠心肌能量代谢重塑,从而保护心功能。更多还原

【Abstract】 Objetives: To observe the effect of Xinfukang(XFK)oral liquid on hemodynamics、cardiac function and the content of energy metabolism material in the rat model of Heart Failure post Myocardial Infarction.Methods: The AMI models were established by ligating left anterion descending coronary artery and the rats with AMI were ramdonly divided into Sham group、Model group、Captopril group ,Yiqihuoxue chinese medicine into XFK high dose group and XFK low dose group.Twenty-four hours after establishing models the rats were fed on XFK oral liquid through the gastrotube for 8weeks. Captopril group was serrved as control,After 8 weeks of the treatment,Hemodynamics、cardiac function and the content of energy metabolism material were measured.Results:(1) The effect of Xinfukang(XFK)oral liquid on hemodynamics of Heart Failure post Myocardial Infarction in rats Contrast with sham group, diastolic blood pressure in model rats was lowered, with statistically significant difference (P<0.05),heart rate and blood pressure change were not significant (P>0.05);compared with model group, heart rate and diastolic blood pressure changes were not statistically significant both in XFK group and Captopril group,but systolic blood pressure in Captopril group was decreased significantly,with statistically very significant difference (P <0.01)。(2) the effect of Xinfukang(XFK)oral liquid on cardiac function of Heart Failure post Myocardial Infarction in rats Contrast with sham group,left end-diastolic pressure(LVEDP) was obviously increased , left ventricular systolic pressure(LVSP)、cardiac output(CO),as well as maximum rate of rise and fall of left systolic pressure(+dp/dtmax,-dp/dtmax) in model group were signififcantly decreased,with statistically very significant difference (P <0.01).Compared with model group,left end-diastolic pressure(LVEDP) was obviously decreased , left ventricular systolic pressure(LVSP)、cardiac output(CO),as well as maximum rate of rise and fall of left systolic pressure(+dp/dtmax,-dp/dtmax) were signififcantly increased in XFK group and Captopril group,with statistically very significant difference (P <0.01).(3)The effect of Xinfukang(XFK)oral liquid on the content of energy metabolism material of Heart Failure post Myocardial Infarction in rats Compared with sham group,the contentl of ATP in left ventricular muscle was obviously decreased,but ADP and AMP were significantly increased in model group(p<0.01).Compared with model group ,the content of ATP was obviously increased in XFK oral group and Captopril group,but ADP, AMP were significantly decreased,with statistically very significant diference(P <0.01).Conclusion:(1) XFK oral liquid can effectively reduce LVEDP, increase LVSP of Heart Failure post Myocardial Infarction in rats,but has no effect on SBP and DBP,thus to improve hemodynamics steady in rats with heart failure post Myocardial Infarction.(2) XFK oral liquid can effectively improve -dp/dtmax、+dp/ dtmax,and CO, so as to improve left ventricular systolic and diastolic function in rats of heart failure after myocardial infarction.(3) XFK oral liquid can effectively improve the content of ATP ,reduce the content of AMP and ADP in left ventricular muscle,thus improve energy metabolism disorder of heart failure after myocardial infarction in rats, in order to protect cardiac function.更多还原