推拿对坐骨神经损伤大鼠行为学和形态学的影响

【作者】 韩东伟；

【导师】 于天源；

【作者基本信息】 北京中医药大学 ， 针灸推拿学， 2008， 硕士

【摘要】 坐骨神经痛是临床中常见的外周神经损伤疾病,给患者带来了极大的痛苦。有关外周神经损伤及坐骨神经损伤的基础研究也不断获得新突破。而传统中医推拿,作为治疗坐骨神经痛的有效手段,其机理却一直没有揭示清楚。因此,研究推拿的作用机理具有一定的理论和临床意义。基于以往课题研究,本课题采用SD大鼠为受试对象,采用坐骨神经钳夹损伤造模方法,暴露大鼠右侧坐骨神经,在其分叉处上方约0.5cm处,用小号持针器夹持10s,造成损伤。对各组进行不同的干预方法3周后,从行为学和病理学的角度进行初步的分析探讨,以冀对后继实验打下基础。本课题的研究内容主要有以下两部分:行为学:Tarlov评分、斜板试验、坐骨神经功能指数、胫神经功能指数、腓总神经功能指数形态学:分别对坐骨神经、脊髓、肌肉进行HE染色和KB染色,观察各组动物切片的差别,为临床疗效提供病理学支持。结论:通过对各组动物实验结果的比较得出:推拿对坐骨神经痛有确切的疗效,在病理学方面可以得到支持。综上所述,推拿对慢性坐骨神经痛大鼠有治疗作用,在行为学上有明显的改善作用,对外周神经和中枢神经的修复再生都有促进作用。更多还原

【Abstract】 The sciatica is clinical common circumference neurotrosis disease, and has brought enormous pain to the patient. The related circumference neurotrosis and the sciatic nerve damage basic research also unceasingly obtain the new breakthrough. But the traditional Chinese medicine massage, as an effective method of treatment for the sciatica, its mechanism always does not have the revelation to be clear actually. Therefore, the research of massage action mechanism has certain theory and clinical significance.Based on former topic research, this topic uses the SD big mouse as the objects who receive tries. We use clamping method to creat the sciatica models: make an incision into the right rear leg; exposes the right sciatic nerve; supports on both sides 10s with the trumpet porte-aiguille above its furcation place approximately 0.5cm place. Carry on different intervention methods to each group for 3 weeks. And hope to carry on the preliminary analysis from the behavior study and pathology angle,In order to build the foundation to the successor experiments.This topic research content mainly has following two parts:The behavior study: Tarlov score, slanting board test, sciatic nerve function index, tibial nerve function index, common peroneal nerve function index.The morphology: HE dyeability for sciatic nerve, medulla spinalis, muscles, and KB dyeability sciatic nerve. Observe the difference of animal slices,and provide the pathology support for the clinical curative effect.Conclusion: The massage has the accurate curative effects,which can be supported from the experiment of behavior and the pathology through the comparation of animal test results.In a word,the massage has the treatment function to the chronic sciatica big mouse,and has the distinct improvement function in the behavior study.It has the promoter action to the circumference nerve and the central nervous system repair regeneration.更多还原