【作者】 聂卫卓；

【导师】 贝丽霞；

【作者基本信息】 黑龙江八一农垦大学 ， 作物栽培学与耕作学， 2008， 硕士

【摘要】 本研究以健康成株的蝴蝶兰花梗腋芽为外植体,采用单因素、二因素完全随机试验设计获得蝴蝶兰无菌苗,筛选出丛生芽途径进行快繁的各阶段的最适培养基;以无菌苗的叶片为外植体采用单因素、二因素完全随机试验设计诱导出类原球茎,进一步分化成无菌苗,筛选出叶片诱导类原球茎进行快繁的各阶段的最适培养基,并且对叶片褐变控制进行研究。研究结果如下:1、蝴蝶兰诱导丛生芽快繁途径各阶段最适培养基(1)中部腋芽为最佳部位,萌芽率为43.33%。(2)花梗腋芽诱导丛生芽最适培养基为1/2MS+6-BA5.0mg/L+NAA0.5mg/L+椰乳10%,诱导率达80%,褐变率低。(3)丛生芽的继代增殖培养基仍沿用诱导培养基,增殖系数为2.89,丛生芽颜色翠绿,生长健壮。(4)无菌苗生根壮苗最适培养基为1/2MS+6-BA3.0mg/L+NAA0.5mg/L,生根率100%,平均生根数4.18条,平均叶片数5.23片。2、叶片诱导类原球茎快繁途径各阶段最适培养基(1)叶片诱导类原球茎最适培养基为VW+6-BA5.0mg/L+椰乳10%,诱导率为54.44%。诱导的类原球茎个大、饱满,生长健壮,有利于增殖。(2)类原球茎增殖培养基为1/2MS+6-BA3.0mg/L+NAA0.3mg/L+香蕉汁200g/L,增殖系数为3.41。增殖速度快,类原球茎颜色翠绿,表面湿润,排列疏松。(3)类原球茎分化培养基为1/2MS+6-BA3.0mg/L+NAA0.4mg/L+香蕉汁200g/L,分化整齐一致,移栽成活率高。(4)其它因素对增殖的影响:液体培养、薄层切割、10个类原球茎切块均有助于增殖。3、对蝴蝶兰的褐变控制进行研究,结果如下:(1)对叶片进行10d黑暗预处理,褐变率降至为48.60%。(2)添加不同种类和浓度的抗褐变剂,均可降低褐变率,以添加2g/LAC效果最好,褐变率降至35.80%,且不影响类原球茎的诱导。(3)对培养材料进行热激处理1h,褐变率降至45.17%,培养材料长势良好。更多还原

【Abstract】 In this study,Phalaenopsis culturing-plants induced by peduncle buds as explants from healthy and adulting plants were get in single and double factors’randomized absolutely experiment designs,the best culture mediums in different stages were selected; PLB were induced by young leaves from culturing-plants in single and double factors’randomized absolutely experiment designs and had grown into culturing-plants and best culture mediums in different stages were selected.The research which was on the leaves’control- browning were taken.The main results were as follows:1、The better culture mediums of inducing tufty buds in different stages are:(1)The buds which is in the middle are the best explants,germination rate is 43.33%。(2)The best culture medium which induces tufty buds is1/2MS+6-BA5.0mg/L+NAA0.5mg/L+ CM10%,the inducing rate is 80%,the browning rate is lower。(3)The best culture medium which proliferates tufty buds is the same as the inducing one,the proliferation is 2.89,the tufty buds are healty and green。(4)The best culture medium which is rooting and sronging is 1/2MS+6-BA3.0mg/L+NAA0.5 mg/L,the rooting rate is 100%,the averaging roots are 4.81 and the averaging leaves are 5.23。2、The best culture mediums of inducing PLB from leaves in different stages(1)The best culture medium which induces PLB is VW+6-BA5.0mg/L+CM10%,the inducing rate is 54.44%。PLB are big、full、healthy and propitious to proliferate。(2)The best culture medium which proliferates PLB is 1/2MS+6-BA3.0mg/L+NAA0.3mg/L+ Banana Juice200g/L,proliferation is 3.41.PLB proliferated rapidly and arranged lossenly,PLB’s colour is green and surfaces are wet。(3)The best culture medium which differents PLB is 1/2MS+6-BA3.0mg/L+NAA0.4mg/L+ Banana Juice200g/L,the PLBS different trimly,rate of survival is improved.(4)The effects of other factors on proliferation:The liquid-culturing、incising in lamella、block in 10 PLBs are propitious to proliferation。3、The results of control-browning are as follows:(1)The darking pretreatment as long as 10 days can reduce browning rate to 48.60%。(2)Different kinds and concentration of Resist-brownings can reduce browning rate, 2g/LAC is the best one,browning rate is 35.80% and there is no bad influence to inducing PLB。(3)The heating pretreatment as long as 1 hour can reduce browning rate to 45.17%,the culturing materials are all right。更多还原