【作者】 孙永贵；

【导师】 黄大鹏；

【作者基本信息】 黑龙江八一农垦大学 ， 动物营养与饲料科学， 2008， 硕士

【摘要】 为研究不同中草药组方及其不同剂量与益生素组成的合生元对早期断奶仔猪的饲喂效果,试验选择28日龄断奶的杜×长×大仔猪72头,根据体重相近、公母各半的原则,随机分为8组,对照一组为空白对照组和对照二组为益生素对照组,试验一、二、三组为合生元一添加组,试验四、五、六组为合生元二添加组,研究合生元添加剂对仔猪生产性能、营养物质表观消化率和肠道微生物的影响。试验结果显示:1.中草药组方筛选采用体外培养基培养微生物的方法研究不同中草药组方、不同浓度添加剂量对猪源乳酸杆菌、双歧杆菌的益菌作用及对大肠杆菌抑菌作用的影响。结果表明,组方2在1:20浓度时对乳酸杆菌的增值效果最好,组方4在1:10浓度时对双歧杆菌的增值效果最好;组方5在1:10浓度时对大肠杆菌的抑菌作用最强。经主成分分析中草药组方的益菌、抑菌效果,最终选择组方2、4用于组成合生元。2.生产性能益生素和中草药添加量分别为0.5%、0.3%时取得了理想的ADG和F/G,此时ADG为626.1g、F/G为1.62,与对照一组相比ADG提高183.9g(P<0.01)、F/G降低0.24(P<0.01)。3.营养物质表观消化率DE、CP、Ca、P各试验组与对照一组相比试验五组最优,分别提高14.14%(P<0.01)、5.91%(P<0.05)、9.97%(P<0.01)和11.10%(P<0.01)。EE、ADF各试验组与对照一组相比试验三组最优,分别提高12.30%(P<0.05)、5.33%(P<0.01)。4.肠道微生物检测合生元能快速促进肠道微生态平衡。在提高乳酸杆菌含量方面,试验三组含量最高,与其余各组相比差异极显著(P<0.01);在双歧杆菌含量方面,益生素对照组及各试验组均高于对照一组,差异显著(P<0.05);在大肠杆菌方面,对照一组含量高于其余各组,与其余各组相比差异极显著(P<0.01);在沙门氏菌方面,对照一组的含量与益生素对照组及益生素对照组与试验一组相比差异不显著(P>0.05),与其余各组相比差异极显著(P<0.01)。更多还原

【Abstract】 An experiment was conducted to study the effect of probiotic and Chinese herbal medicine with different chroma on piglets weaned early. 72 weaned piglets aged 28 days were divided randomly into 8 groups: fed control diet (synbiotics 1), control diet+probiotic (synbiotics 2), control diet+ synbiotics 1 (trial groups 1, 2, and 3), control diet+ synbiotics 2 (trial groups 4, 5, 6). The effect of growth performance, AD of feed and microbe in intestines was studied. The results showed:1. The recipe’s screening of Chinese herbal medicine: An experiment was conducted to study the effect of different combination and dose. The results shows that second prescription had the best effect on Bacillus lactobacilli by the chroma of 1:20; the forth one has the best effect on bifidus by the chroma of 1︰10, the fifth one has the best restrain on Escherichia coli by the chroma of 1︰10. Finally, the synbiotics was composed by the forth and second prescription according to the principal component analysis of all the prescriptions.2. Growth performance: The best ADG and F/G is gotten when the addition of probiotic. Chinese herbal medicines are 0.5% and 0.3% compared with two groups fed by control diet (CK): the advance of ADG is 183.9 (p<0.01), the drop of F/G is 0.24 (p<0.01), which is 626.1g, 1.62.3. AD of nutrient substance: The fifth experiment group is the best and the index of DE, CP, Ca, and P is higher by 14.14%, 5.91%, 9.97% and 11.10% than the first CK. The index of EE and ADF of the third experiment group is higher than the first CK by 12.30% (P<0.05) and 5.33% (P<0.01).4. The microbiological detection in intestines: The synbiotics can balance the micro zoology of intestines. The third group is the best which contained the most lactobacilli and has the significant difference with others. The index of Bacillus bifidus in the second CK and the other groups is significant higher than the first CK (P<0.01); however, the index of Escherichia coli in the first CK is significant higher than other groups (P<0.01); also in which group, there is the highest index of salmonella and no significant difference (P>0.05) with the second CK and also this difference existed between the first trial group and the second CK but significant difference (P<0.01) with all others.更多还原