【作者】 田志杰；

【导师】 周义发；

【作者基本信息】 东北师范大学 ， 生物化学与分子生物学， 2008， 硕士

【摘要】 目的:正交法确定黑木耳粗多糖的最佳提取条件,并研究其体外清除自由基和对血清SOD活性的影响。方法:采取四因素三水平正交试验设计L9(34)并结合SPSS13.0软件进行分析;超氧阴离子自由基(O2-)由邻苯三酚自氧化法产生,采用Fenton体系产生羟自由基(·OH) ,通过检测(O2-)和(·OH)含量的变化,来研究木耳粗多糖对O2-和·OH的清除作用;通过TBA比色,研究木耳多糖对肝脏过氧脂质化的作用;实验鼠按200mg/kg体重灌胃给木耳粗多糖提取液,通过其血清中SOD对邻苯三酚自氧化的抑制作用,研究木耳多糖对血清中SOD活性的影响。结果:木耳多糖的最佳提取条件是:料水比为1:45,提取温度为80oC,水提时间1小时,提取次数3次,得率4.64%;木耳粗多糖在体外能清除O2-和·OH ,在0-0.2mg/ml清除率逐渐上升;在0-0.5mg/ml内,木耳多糖队肝脏的过氧脂质化抑制逐渐增强;灌胃后血清SOD对邻苯三酚自氧化抑制率明显提高,说明活性明显增强。结论:木耳粗多糖具有在体外清除超氧阴离子自由基(O2-)、羟自由基(·OH)、抑制肝脏的过氧脂质化、提高血清SOD活性的作用。更多还原

【Abstract】 Objective To study the extration optimization of polysaccharides from Auricularia auricular using uniform design and the effects of Auricularia auricular polysaccharides on scavenging oxygen free radical and anti-lipid peroxidation in vitro and the activity of SOD in serum.Methods Uniform Design was used to optimize the effects of processing parameters of extraction from Auricularia auricular on the yield of polysaccharides. Four independent variables such as extraction time (hour), solid:liquid ratio (g/ml), extraction times and extraction temperature (oC) were studied. The optimal conditions were determined from SPSS13.0. Superoxide anion free radical ((O2-)) induced by the auto-ox idation of pyrogallol and hydroxyl free radical (·OH ) induced by Fenton system were used to study the effect on scacenging oxygen free radical.The inhibition on lipid peroxidation malondialdehyde (MDA )of rat liver was determined by thiobarabituric acid (TBA ) colorimetric method. The rats were fed with polysaccharides from Auricularia auricular orally at the dose of 200mg/kg, then the contents of SOD in the serum of the rats were determined.Results The optimum conditions of polysaccharides extraction were liquid:solid ratio of 1:45, extraction time of 1 hour, water extraction temperature of 80oC, and water extraction times of 3 times. Under optimized conditions, the experimental yield4.3 % agreed closely with the predicted yield. Auricularia auricular polysaccharides could significantly scavenge (O2-)and·OH, and remarkably inhibit the lipid peroxidation of rat liver. The scavenging and inhibitory effect exhibited in a concentration-dependent manner in a specific range. It also significantly increased the contents of SOD in serum of rats after gastric perfusion.Conclusion The results indicated that possesses the effect of scavenging oxygen free radicals and anti-lipid peroxidation, which shows the protection of liver mitochondria.更多还原