【作者】 陈海伟；

【导师】 王丽；

【作者基本信息】 东北师范大学 ， 遗传学， 2008， 硕士

【摘要】 植物组织培养是指在离体条件下利用人工培养基对植物器官、组织、细胞、原生质体等进行培养,使其形成完整的植株。其原理是利用植物细胞的全能性使植物成熟细胞在脱分化之后再分化,形成愈伤组织,由愈伤组织再形成完整的植株。二色补血草(Limonium bicolor)是蓝雪科( Plumbaginaceae)补血草属的越年生草本植物。利用种子萌发产生的无菌苗进行组织培养,建立完善的二色补血草无性繁殖体系,是对植物组织培养的完善和发展。利用组织培养技术进行离体保存,可大大节省人力、物力及土地,它对于植物遗传多样性的保护,人类生存环境改善,维持农牧业稳定持续发展都具有很重要的意义。本实验分别探讨:不同激素的配比对以无菌苗的子叶、胚轴为材料诱导愈伤组织的影响;不同激素的配比对愈伤组织增殖及不定芽分化的影响;不同激素的配比对生根的影响;炼苗和移栽。经过实验,取得以下结果:1.培养基MS+6-BA(0.3-0.7mg/L)+NAA(0.1-0.5 mg/L)均可诱导二色补血草种子萌发产生的无菌苗的子叶产生愈伤组织及不定芽,其中激素诱导的最适配比有0.3 mg/L 6-BA +0.5 mg/L NAA、0.5 mg/L 6-BA+0.1 mg/L NAA和0.7 mg/L 6-BA+0.1 mg/L NAA三个组合。2.培养基MS+6-BA(0.3-0.7 mg/L)+NAA(0.1-0.5 mg/L)均可诱导二色补血草种子萌发产生的无菌苗的胚轴产生愈伤组织及不定芽,其中最适激素配比是0.3 mg/L 6-BA+0.5 mg/L NAA;诱导时间长,对不定芽分化有利。3.子叶更适合作为愈伤组织诱导及不定芽分化的实验材料。4. MS0、MS +0.2 mg/L NAA和1/2 MS0均可诱导二色补血草不定芽生根,其中最佳培养基是MS0培养基,不定芽生根不需要激素NAA或对激素NAA要求较低。更多还原

【Abstract】 Plant cells can be grown in isolation from intact plants in tissue culture systems. The cells have the characteristics of callus cells, rather than other plant cell types. The theory "plant cell totipotency" indicates that plant regeneration from single cell culture can be accomplished through the redifferentiation of the callus.Limonium bicolor (Plumbaginaceae) belongs to biennial forb growing in the typical steppe. It is improvement and development of Limonium bicolor tissue culture system that aseptic seedlings are used as materials. The technology is so significant for conservation of genetic diversity and living environment. It can save a great deal of manpower and material resources, improve the efficiency and quality of agricultural development. This study investigated the regeneration ability of callus from cotyledon and embryonic axis under different hormone conditions. Experimental results were as follows:1. On MS medium containing 6-BA(0.3-0.7mg/L)and NAA(0.1-0.5 mg/L), callus and adventitious bud were induced from cotyledon. The best hormone combinations were 0.3 mg/L 6-BA +0.5 mg/L NAA, 0.5 mg/L 6-BA+0.1 mg/L NAA and 0.7 mg/L 6-BA+0.1 mg/L NAA;2. On MS medium containing 6-BA(0.3-0.7mg/L)and NAA(0.1-0.5 mg/L), callus and adventitious bud were induced from embryonic axis. The best combination was 0.3 mg/L 6-BA+0.5 mg/L NAA. The longer induction time, the better differentiation of adventitious bud;3. Cotyledon was much better than adventitious bud as material of inducing callus and adventitious bud;4. MS0 medium MS medium with 0.2 mg/L NAA and 1/2 MS0 medium could induce rooting of adventitious bud, the best was MS0 medium. During this course, the requirement for NAA was at low level.更多还原