【作者】 闫磊；

【导师】 罗国容；

【作者基本信息】 广西医科大学 ， 人体解剖与组织胚胎学， 2008， 硕士

【摘要】 目的:检测人类动力素蛋白(kinectin)D2与D3选择剪接区(alternative splicing site)在人肝细胞癌(HCC)组织与癌旁组织以及正常肝组织中表达的差异性,探索kinectin的选择剪接区与肝癌发生之间可能存在的联系。方法:用RT-PCR法获取45例肝癌组织标本和癌旁组织标本以及10例正常肝组织标本的cDNA,并用半定量PCR法分别检测其D2选择剪接区(以下简称D2区)和D3选择剪接区(以下简称D3区)表达情况的差异性;将MBP-kinectin融合蛋白作为抗原,通过ELISA技术检测45例肝癌患者血清标本中kinectin抗体的阳性率,并进行统计学分析。结果:肝癌组织D2~L/D2~S比率高于癌旁组织和正常肝组织,差异有统计学意义;D3~L/D3~S比率在肝癌组织、癌旁组织及正常肝组织之间无明显差异;肝癌组织D2~L/D2~S比率与血清中kinectin抗体滴度值无明显相关性。结论:包含D2区的kinectin异型体在肝癌组织中表达水平增高,提示其可能与肝癌的发生有一定的相关性。更多还原

【Abstract】 Objective: Analyze the difference in expression of D2 and D3 alternative splicing sites of human kinectin in human hepatocellular carcinoma(HCC)tissues, adjacent non-cancerous tissues and normal liver tissues,and investigate the possible relationship between alternative splicing sites of kinectin and the hepatocarcinogenesis.Methods: The cDNA was obtained by RT-PCR in 45 coupled HCC cancerous and adjacent tissues,and 10 normal liver tissues.The difference in the expression of D2 and D3 alternative splicing sites in cDNA was examined by semi-quantitative PCR,fusion protein MBP-kinectin which was obtained by bio-engineering was used as the antigen. The positive rate of anti-kinectin in hepatocellular cancerous serum of 45 patients were detected by ELISA.Then,statistical analysis was performed.Results: The ratio of D2~L/D2~S in hepatocellular cancerous tissues is higher than that of adjacent non-cancerous tissues and normal liver tissues.There isstatistical significance between them.The ratio of D3~L /D3~S has no Significant difference among hepatocellular cancerous tissues ,adjacent non-cancerous tissues and normal liver tissues.There is no obvious correlation between the ratio of D2~L/D2~S in hepatocellular cancerous tissues and the titers of anti-kinectin in coupled serum by ELISA.Conclusion: Variant containing D2 is overexpressed in cancerous tissues and this alteration may be tumor associated.更多还原