【作者】 吾甫尔；

【导师】 赛力克·马高维亚；

【作者基本信息】 新疆医科大学 ， 外科学， 2008， 硕士

【摘要】 目的观察不同剂量低分子肝素钠对急性DVT大鼠在不同时期治疗后血管形态学的变化。方法将72只SD大鼠制备模型后,随机按血栓形成后1、3、7、14天分期,每期18只,分为Ⅰ期、Ⅱ期、Ⅲ期、Ⅳ期。然后每期再根据低分子肝素钠剂量分为:低分子肝素钠小剂量组(700U/kg、一次/24h,n=6)、低分子肝素钠大剂量组(700U/kg、一次/12h,n=6)、模型组(0 U/kg,n=6)。低分子肝素钠大、小剂量组均由大鼠腹壁皮下注射,连续7日。实验结束后获取病变段血管,观察内皮细胞数量变化,胶原阳性染色面积百分比变化,并与同期模型组进行比较。结果模型组在Ⅱ期、Ⅲ期分别死亡一只,低分子肝素钠治疗大剂量组Ⅰ期、Ⅲ期、Ⅳ期内死亡4只。在Ⅰ期低分子肝素钠大、小剂量组内皮细胞数量与模型组相比均有统计学差异(P<0.05),组间亦有差异.低分子肝素钠大、小剂量组胶原阳性染色面积百分比与模型组相比均有统计学差异(P<0.05),组间相比无差异;在Ⅱ期,低分子肝素钠大、小剂量组内皮细胞数量与模型组相比均有统计学差异(P<0.05),组间无差异;在Ⅲ期、Ⅳ期低分子肝素钠大、小剂量组内皮细胞数量与模型组相比无统计学差异(P>0.05);在Ⅱ期、Ⅲ期低分子肝素钠大、小剂量组胶原阳性染色面积百分比与模型组相比仍有统计学差异(P<0.05),组间亦有差异;在Ⅳ期低分子肝素钠大、小剂量组胶原阳性染色面积百分比与模型组相比无统计学差异(P>0.05)。结论血栓形成3天内,大、小剂量低分子肝素钠对内皮细胞均有保护作用,以大剂量效果较好。血栓形成7天内,大、小剂量低分子肝素钠对对胶原增生均有抑制作用,随着时间的延长,以大剂量低分子肝素钠抑制作用最强。更多还原

【Abstract】 Objective To observe the effect of different dose,s Low molecular weight heparin( LMWH) on morphological changes of vein wall after differenct periods of deep vein thrombosis (DVT) in rats. Methods Firstly,72 sprague-dawley (SD) rats were randomly divided intoⅠ、Ⅱ、Ⅲ、Ⅳperiods on 1st,3rd,7th,14th day following thrombosis respectively, there were 18 SD rats in every period.Then the 18 SD rats in each period were separated to 4 group. Low dose’s Low molecular weight heparin( LMWH) group (700u/kg,1t/24h, n=6.)、high dose’s Low molecular weight heparin(LMWH) group(700u/kg, 1t/12h, n=6.)、thrombosis group(0,000u/kg, n=6). In each group, to observe the remain quantity of endothelial cells and the expression areas of collagen by dyeing. Results 2 SD rats in thrombosis group were died inⅡ、Ⅲperiod respectively. 4 SD rats in high dose’s Low molecular weight heparin( LMWH) group were died inⅠ、Ⅲ、Ⅳperiod in thrombolytic therapy. InⅠperiod, the quantity of endothelial cells in low、high dose’s LMWH group have significant difference with thrombosis group respectively (P<0.05). The difference exisited in interclass as well(P<0.05). InⅠperiod, the expression areas of collagen in low、high dose’s LMWH group have significant difference with thrombosis group respectively (P<0.05); InⅡperiod, the quantity of endothelial cells in low、high dose’s LMWH group have significant difference with thrombosis group respectively (P<0.05) ,The difference do not exisited in interclass (P>0.05);InⅢ、Ⅳperiods,the quantity of endothelial cells in low、high dose’s LMWH group haven,t significant difference with thrombosis group respectively(P>0.05). InⅡ、Ⅲperiod, the expression areas of collagen in low、high dose’s LMWH group have significant difference with thrombosis group respectively (P<0.05), The difference exisited in interclass as well(P<0.05)。InⅣperiod, The expression areas of collagen in low、high dose’s LMWH group haven,t significant difference with thrombosis group respectively (P<0.05). Conclusions Different dose,s LMWH have protective effect on the quantity of endothelial cells during 3 days after thrombosis, the effect of high dose’s LMWH is better; Different dose,s LMWH haven,t protective on the quantity of endothelial cells after 7 days following thrombosis,but they have inhibitary effect on the expression areas of collagen in every period, the effect of high dose’s LMWH is better.更多还原