【作者】 杜靖；

【导师】 海米提·阿布都力木； 帕尔哈提·阿布都热依木；

【作者基本信息】 新疆医科大学 ， 生理学， 2007， 硕士

【摘要】 目的:研究宫颈炎组织和宫颈癌组织中MHC-Ⅰ类抗原呈递相关蛋白（TAP₁、TAP₂、β2-MG、HSP70）的表达在维吾尔族和汉族妇女中的关系。初步探讨维吾尔族宫颈癌发病及发展过程中MHC-Ⅰ类抗原呈递相关蛋白TAP₁、TAP₂、β2-MG和HSP70在抗原呈递过程中的作用及影响因素。方法:应用免疫组织化学方法和聚合酶链反应（PCR）技术检测87例宫颈炎组织（维吾尔族36例,汉族51例）以及142例宫颈癌组织（维吾尔族99例,汉族43例）中HPV抗原呈递相关蛋白TAP₁、TAP₂、β2-MG和HSP70的表达和HPV16的感染情况。结果:TAP1、TAP2和β2-MG在宫颈癌组织中阳性表达率（51.61%、60.0%、61.42%）,均低于宫颈炎组织（78.20%、94.81%、96.00%）,（P＜0.05）。在宫颈癌组织中,汉族TAP1、TAP2和β2-MG阳性率分别为33.33%、42.11%和78.95%,维吾尔族TAP1、TAP2和β2-MG阳性率分别为59.09%、67.82%和53.93%。两组相比差异具有统计学意义（P＜0.05）。在宫颈炎组织中,汉族TAP1、β2-MG阳性率分别为70.21%和93.48%,维吾尔族TAP1、β2-MG阳性率分别为90.32%和100%。两组相比差异具有统计学意义（P＜0.05）。β2-MG与维吾尔族宫颈癌HPV16关系为正相关（r=0.216,P＜0.05）。汉族宫颈癌组织中,TAP2与β2-MG间存在正相关（r=0.331,P＜0.05）。维吾尔族宫颈癌组织中,TAP1与β2-MG之间、TAP1与HSP70之间存在正相关（r=0.351,P＜0.05;r=0.224,P＜0.05）。结论:宫颈癌组织中TAP1、TAP2和β2-MG的表达量下降,且在维吾尔族和汉族间相关性表达呈现差异。提示不同蛋白在不同民族间宫颈癌的辅助诊断方面可能存在不一致。更多还原

【Abstract】 Objective:study on the expression of MHC class I antigen presentation associated proteins TAP1, TAP2,HSP70,andβ2-MG in cervical cancer lesions and its correlation with HPV16 infection in cervical cancer patients of Uighur and Han women..Methods:We applied S-P Immunohistochemistry and PCR to detect the expression of proteins TAP1,TAP2,HSP70,β2-MG and infection of HPV16 respectively in 87 cervicitis tissues from 51 Han and 36 Uighur,women and 142 cervical cancer lesions from 43 Han and 99 Uighur women.Results:The expression rates of TAP1、TAP2、β2-MG in cervical cancer tissues from both Han and Uighur women(51.61%,60.0%, 61.42%,respectively) were significantly reduced when compared to that(78.20%,94.81%,.96.00%, respectively,and p＜0.05) in cervicitis tissues from both ethnic groups.Among the cervical cancer tissues,the expression rate of TAP1、TAP2、β2-MG of Han women were 33.33%,42.11%,and 78.95%respectively,whereas the extression rate for these proteins of Uighur women were 59.09%, 67.82%,and 53.93%respectively,and the differences were significant between Han and Uighur women(p＜0.05).In cervicitis tissues,the expression rate of TAP1 andβ2-MG were significantly different(p＜0.05) between Han and Uighur women,that were 70.21%and 93.48%in former,and 90.32%and 100%in latter respectively.The expression rate ofβ2-MG in cervical cancer lesions was positively correlated with infection of HPV16 in Uighur women(r=0.216,p＜0.05).There was positive correlation in expression ofβ2-MG and TAP2 in cervical cancers of Han women(r=0.331, p＜0.05),and there were also positive correlations between expression ofβ2-MG and TAP1 and TAP1 and HSP70 in cervical cancers of Uighur women(r=0.351,p＜0.05 and r=0.224,p＜0.05, respectively).Conclusion:The expression of TAP1,TAP2 andβ2-MG in the cervical cancer lesions was reduced compared to the cervicitis tissues,and there were conelation differences in expression of these proteins between Han and Uighur women.This suggested possible discrepancies between different ethnic groups when considering the diagnostic significance of these protein expressions in cervical cancer lesions.更多还原