【作者】 王福新；

【导师】 丁剑冰；

【作者基本信息】 新疆医科大学 ， 免疫学， 2008， 硕士

【摘要】 目的观察柴虎疏肝煎剂对肝纤维化大鼠肿瘤坏死因子-α(tumor necrosis fac-tor-α,TNF-α)和干扰素-γ(interferon-γ,INF-γ)的影响,并且观察该药对大鼠肝纤维化治疗作用及意义。方法80只Wistar大鼠随机分为正常组、模型组组、中药金三莪预防组及治疗组、柴虎疏肝煎剂预防组、柴虎疏肝煎剂低、中、高剂量治疗组,共8组,每组10只。除正常组以外,其余各组以40%四氯化碳(Carbon tetrachloride,CCL4)石蜡油造模,中药金三莪预防组和柴虎疏肝煎剂预防组在造模同时分别给予中药金三莪和柴虎疏肝煎剂。造模4周后灌胃治疗,各治疗组分别给予相应剂量的中药治疗8周。病理切片观察各组大鼠肝纤维化程度,免疫组织化学技术检测两种细胞因子在组织标本中的表达,利用酶联免疫吸附法(ELISA)检测各组血清中TNF-α和INF—γ的浓度。结果病理学结果:与正常组相比,模型组及柴虎疏肝煎剂低剂量组肝纤维化明显,差异有统计学意义(P<0.01)。与模型组相比,柴虎疏肝煎剂中、高剂量组肝纤维化程度明显减轻(P<0.05)。免疫组化结果:与正常组相比,模型组、柴虎疏肝煎剂低剂量组INF-γ表达明显增强(P<0.01)。与模型组相比,中药金三莪治疗及预防组,柴虎疏肝煎剂预防、中、高剂量治疗组INF-γ表达明显减弱(均为P<0.01)。与正常组相比,模型组和柴虎疏肝煎剂低剂量治疗组TNF-α表达明显增强(P<0.05)。与模型组相比,中药金三莪预防及治疗组、柴虎疏肝煎剂预防及中、高剂量组TNF-α表达明显减弱,(P<0.05、P<0.01、P<0.05、P<0.05、P<0.01)。血清学结果:与正常组相比,模型组血清中IFN-γ的浓度明显升高(P<0.01),与模型组相比,柴虎疏肝煎剂中、高剂量治疗组血清中IFN-γ浓度明显降低(P<0.01)。与正常组相比,模型组血清中TNF-α浓度明升高(P<0.01)。与模型组相比,中药金三莪预防组及治疗组、柴虎疏肝煎剂预防组、柴虎疏肝煎剂低、中、高剂量治疗组血清中TNF-α浓度明显降低(P<0.05、P<0.01、P<0.05、P<0.05、P<0.01、P<0.01)。结论柴虎疏肝煎剂可能主要是通过降低肝纤维化大鼠血清中细胞因子IFN-γ和TNF-α的浓度,并且降低TNF-α和IFN-γ在肝纤维化大鼠肝组织中的表达,进一步抑制肝脏的炎症反应,减轻肝脏损伤而起到治疗肝纤维化的作用,为临床中医用药提供实验依据。更多还原

【Abstract】 Objective To observe the effect of ChaiHu ShuGan decotion on cytokines tumor necrosis factor-α(TNF-α)、interferon-γand observe the effect of this Traditional Chinese Medicine on rats with hepatic fibrosis.Method The eighty rats were divided into eight groups randomly, every group included ten rats. They were control group、model group、Traditional Chinese Medicine JinSanE preventive group、ChaiHu ShuGan decotion preventive group、Traditional Chinese Medicine JinSanE therapeutic group、ChaiHu ShuGan decotion low dosage、middle dosage、high dosage therapeutic group. Except control group, the other groups were given 40% carbon tetrachloride liquid paraf-fin to make model of hepatic fibrosis . Traditional Chinese Medicine JinSanE preventive group and ChaiHu ShuGan decotion preventive group were administrated Traditional Chinese Medicine JinSanE and traditional Chinese drugs ChaiHu ShuGan decotion re-spectively when the model began to make. Four weeks later , the therapeutic groups were administrated the traditional Chinese drugs JinSanE and ChaiHu ShuGan decotion eight weeks .The concentration of TNF-αand INF-γin the serum of rats in every group were de-tected by enzyme linked immunosorbent assay(ELISA);the degree of hepatic fibrosis of rats was detected by pathological section ;the degree of the two cytokines expressed in liver were detected by immunohistochemistry .Result The result of pathology, com-pared with control group , the degree of hepatic fibrosis of model group and ChaiHu ShuGan low dosage therapeutic groups obviously and had difference significance(P<0.01).Compared with model group ,the degree of hepatic fibrosis of ChaiHu ShuGan de-cotion middle and high dosage group were improved obviously and had difference sig-nificance(P<0.05). The result of immunohistochemistry , compared with control group, the expression of IFN-γin liver of rats of model group and ChaiHu ShuGan decotion low dosage therapeutic groups was enhanced obviously and had difference significance (P<0.01). Compared with model group, the expression of IFN-γin liver of rats of Traditional Chinese Medicine JinSanE preventive group、Traditional Chinese Medicine JinSanE therapeutic groups、ChaiHu ShuGan decotion preventive group、ChaiHu ShuGan decotion m i d d l e a n d h i g h d o s a g e t h e r a p e u t i c g r o u p s w a s w e a kened obviously and had difference significance(P<0.01). Compared with control group, the expression of TNF-αin liver of rats of model group and ChaiHu ShuGan decotion low dosage therapeutic groups was enhanced obviously and had difference significance (P<0.05). Compared with model group, the expression of TNF-αin liver of rats of Tra-ditional Chinese Medicine JinSanE preventive group、Traditional Chinese Medicine therapeutic group、ChaiHu ShuGan decotion preventive group、ChaiHu ShuGan de-cotion middle and high dosage therapeutic groups was weakened obviously and had dif-ference significance(P<0.05、P<0.01、P<0.05、P<0.05、P<0.01).The reslutof serology, compared with control group, the concentration of IFN-γin serum of model group was increased obviously and had difference significance(P<0.01). Compared with model group ,the concentration of IFN-γin serum of ChaiHu ShuGan decotion middle and high dosage therapeutic groups was decreased obviously and had difference sig-nificance (P<0.01). Compared with control group, the concentration of TNF-αin se-rum of model group was increased obviously and had difference significance(P<0.01).Compared with model group ,the concentration of TNF-αin serum of Traditional Chinese Medicine JinSanE preventive group、Traditional Chinese Medicine JinSanE therapeutic group、ChaiHu ShuGan decotion preventive group、ChaiHu ShuGan decotion low dosage、middle dosage、high dosage therapeutic group was decreased obviously and had difference significance(P<0.05、P<0.01、P<0.05、P<0.05、P<0.01、P<0.01).Conclusion ChaiHu ShuGan decotion play a role in improvement of hepatic fibrosis of rats possibly mainly by decreasing the concentration of TNF-αand INF-γin serum and decreasing the expression of TNF-αand IFN-γin liver of rats with hepatic fibro-sis ;and then inhabiting inflammation and decreasing injury of liver. This research hoped to give experimental basis on clinical treatment.更多还原