【作者】 张红霞；

【导师】 王军；

【作者基本信息】 河南大学 ， 中药学， 2008， 硕士

【摘要】 目的:观察生姜水提物对大鼠全脑缺血再灌注炎症级联反应的影响。方法:SD雄性大鼠随机分为假手术组、模型对照组、尼莫地平组和生姜水提物组,灌胃给药,每天1次,共6次。末次给药后1h,采用Pulsinelli’s四动脉阻断法造成大鼠全脑缺血10min再灌注3h损伤模型(CIR)。酶联免疫吸附法(ELISA)测定脑组织白细胞介素-1β(IL-1β)、白细胞介素-6(IL-6)、白细胞介素-8(IL-8)、肿瘤坏死因子-α(TNF-α)和细胞间黏附分子-1(ICAM-1)、血管细胞间粘附分子-1(VCAM-1)、内皮细胞选择素(E-selectin)、血小板选择素(P-selectin)含量。结果:与假手术组比较,模型对照组大鼠脑组织TNF-α、ICAM-1、P-selectin显著升高(P<0.05);其他炎性细胞因子(IL-1β、IL-6、IL-8)和黏附分子(VCAM-1、E-selectin)均有不同程度的升高,但无统计学意义(P>0.05)。生姜水提物能明显降低大鼠脑组织TNF-α、ICAM-1、P-selectin含量(P<0.05～0.01),对其他炎性细胞因子(IL-1β、IL-6、IL-8)和黏附分子(ICAM-1、VCAM-1、E-selectin)均有不同程度的降低作用,但无统计学意义(P>0.05)。结论:炎性细胞因子与黏附分子及由此介导的炎症级联反应参与缺血再灌注性脑损伤的病理过程,生姜水提物治疗脑缺血再灌注损伤的机制之一与降低炎性细胞因子与黏附分子含量、抑制炎症级联反应有关。更多还原

【Abstract】 [Objective] To observe the change of inflammatory cytokines and adhesion molecules content after cerebral ischemia/reperfusion (CIR) and the effect of aqueous extract of ginger.[Methods] Male SD rats were evenly randomized into sham-operation, model, nimodipine, aqueous extract of ginger groups. CIR model was produced by Pulsinelli’s“Four Vessel Occlusion”method. The contents of inflammatory cytokines including interleutin-1β(IL-1β),interleutin-6(IL-6),interleutin-8 (IL-8) and tumor necrosis factor–α(TNF-α) and adhesion molecules including intercellular cellular adhension molecule (ICAM-1), vascular cellular adhension molecule (VCAM-1), endothelium selectin (E-selectin) and platelet selectin (P-selectin) in brain tissue were detected by enzyme-linked immunosorbent assay(ELISA). [Results] Compared with sham-operation group, the contents of TNF-α、ICAM-1and P-selectin in brain tissue of model group increased significantly (P<0.05) . While compared with model group, aqueous extract of ginger could decrease the contents of TNF-α,ICAM-1 and P-selectin in brain tissue(P<0.05～0.01), but no significant difference to the content of IL-1β,IL-6,IL-8, ICAM-1,VCAM-1 and E-selectin, compared with model control group.[Conclusion] The therapeutic action of aqueous extract of ginger on cerebral ischemia reperfusion can be realized by decrease the inflammatory response induced by cytokines and adhesion molecules.更多还原