【作者】 李浩；

【导师】 王志强；

【作者基本信息】 扬州大学 ， 基础兽医学， 2008， 硕士

【摘要】 本实验研究了洛克沙胂对鲫鱼组织Na~+-K~+-ATP酶活性影响及肾细胞DNA损伤程度。发现洛克沙胂对于鲫鱼组织Na~+-K~+-ATP酶活性和肾细胞DNA损伤程度具有直接的影响。首先以4.0、16.0、64.0mg/L洛克沙胂暴露48、96、144h后检测鲫鱼的鳃、肾脏和肝中的Na~+-K~+-ATP酶活性。试验结果表明,在同一组织中,随着处理浓度的提高,洛克沙胂对鲫鱼各组织Na~+-K~+-ATP酶活性抑制作用更为明显,呈现出明显的浓度-效应关系(P<0.01)。对于不同组织,同一处理浓度的洛克沙胂对鲫鱼鳃中Na~+-K~+-ATP酶活性抑制作用更为明显,其次是肝和肾(P<0.01)。在同一浓度下,洛克沙胂对鲫鱼各组织Na~+-K~+-ATP酶活性抑制率呈明显的时间-效应关系,暴露时间越长,抑制率越高(P<0.01)。单细胞凝胶电泳试验(SCGE)结果表明,鲫鱼暴露于0.5、1.0、2.0mg/L洛克沙胂中72h能引起鲫鱼肾细胞明显的DNA损伤,与空白对照相比有显著性差异(P<0.05),并在设定剂量范围内呈现一定的剂量-效应关系;同时,鲫鱼肾细胞于体外分别暴露于1、10、100、500、1000mg/L洛克沙胂3、6、12h后,可引起严重的DNA损伤。可得出结论,无论体内染毒还是体外染毒,洛克沙胂均可致鲫鱼肾细胞DNA明显的断裂和迁移,对肾细胞DNA造成损伤,各染毒组与阴性对照组有显著差异。在实验期间,实验剂量内呈现剂量-效应关系和时间-效应关系。此外,将离体鲫鱼肾细胞于体外分别暴露于1、10、100、500、1000mg/L洛克沙胂的代谢产物亚砷酸钠3、6、12h后,可引起更为严重的DNA损伤,实验剂量内同样呈现剂量-效应关系和时间-效应关系。更多还原

【Abstract】 In order to provide a reference that the influence of Roxarsone on Na~+-K~+-ATPase in crap’s tissue and DNA damage. Firstly, liver, kidney and gill were collected from craps at 48, 96, and 144 hours from Roxarsone exposure group in which craps were exposed to solution containing Roxarsone at the dose of 4.0,16.0,64.0mg/L.After that, the activities of Na~+-K~+-ATPase was analyzed. The result showed that Roxarsone can significantly inhibit the activation of Na~+-K~+-ATPase of crap in liver, kidney and gill. The inhibition of Na~+-K~+-ATPase increased with the concentrateion and the exposure time of Roxarsone,showing dose-response relationship and time-response relationship. Secondly, the results of single-cell gel eiectrophoresi(sSCGE)showed that Roxarsone at the dose of 0.5、1.0、2.0mg/L, could induce DNA damage in crap kidney cells in vivo, especially at high dose doses of exposure, and might have a potentiall genotoxicity. The damage caused by Roxarsone was obviously great and the differentce was significant,compared with those of the control(P<0.05). The extent of DNA damage increased with the concentrateion of Roxarsone, showing a striking dose-response relationship.At the same time, Roxarsone at the dose of 1.0,10,100,500,1000mg/L and exposuring for 3h,6h,12h respectively, could induce DNA damage on crap kidney cells in vitro, The damage caused by Roxarsone was obviously great and the differentce was significant,compared with those of the control(P<0.05). The extent of DNA damage showed a striking dose-response relationship and time-response relationship.更多还原