【作者】 董明；

【导师】 苏德荣；

【作者基本信息】 北京林业大学 ， 草业科学， 2008， 硕士

【摘要】 阿诺红鞑靼忍冬(Lonicera tatarica Arnold Red)是忍冬科落叶灌木优良植物品种,具有极好的景观价值和生态价值。为开发其在天津地区的利用价值,本论文通过盆栽试验,在人工水分胁迫下和人工盐胁迫条件下对阿诺红鞑靼忍冬两年生苗生长状况以及胁迫不同时期叶片各项生理指标进行观测,对其抗旱和耐盐机理进行了初步探讨,并对其抗旱性和耐盐性作出评价,为植物资源的合理利用和可持续发展提供科学依据。同时,本文还对阿诺红鞑靼忍冬扦插繁殖技术进行了研究,以期为其工厂化快速育苗提供提供理论和技术支持。在扦插试验中,结果表明不同外源激素种类和浓度对插条生根率、平均根长、萌芽率有极显著影响,对平均生根数、愈伤率无显著影响。平均生根率以ABT1100 mg.kg-1处理时促进效果最好,最高达到87%;平均根长以浓度为100 mg.kg-1的ABT1处理时效果最好,最长达到46.92mm;萌芽率在用浓度为100mg.kg-1的ABT1处理时最高达到90%。综合来看ABT1比IBA对于促进阿诺红鞑靼忍冬的插穗生根效果稳定。在干旱胁迫试验中,结果表明阿诺红鞑靼忍冬各项生理指标在干旱胁迫下都有所变化,在一定程度上反映了植物对干旱胁迫的调整适应。游离脯氨酸,可溶性糖,过氧化物酶,叶绿素,束缚水含量在干旱胁迫下总体上的变化趋势有利于阿诺红鞑靼忍冬适应干旱逆境,组织含水量虽然呈下降趋势,但在整个干旱胁迫过程中都能维持在一个较高的水平。综合上述指标的表现,说明阿诺红鞑靼忍冬具有较强的抗旱能力。在盐胁迫试验中,结果表明在不同的盐分环境中,阿诺红鞑靼忍冬叶片丙二醛和游离脯氨酸含量表现出不同的变化,阿诺红鞑靼忍冬在低于在4.5g/l的盐分环境中有较强的耐受能力,在盐分环境中胁迫14d后通过自身的调节能适应一定程度的盐分环境。更多还原

【Abstract】 Arnold Red Honeysuckle is a perennial deciduous shrub of Lonicera in Caprifoliaceae; it has good landscape value and ecological value. In order to exploited its utilization value in Tianjin area, by pot experiment under artificial water stress conditions, the author of this paper observed a row physiological index in leaves of the two-year-old Arnold Red Honeysuckle plants for a long continuous-time, discussed on the drought resistance and salt-tolerant mechanism, and evaluated its drought resistance and salt tolerance, which provided scientific basis for reasonable utilization and sustainable development of plant resources. Meanwhile, this paper had study on cutting propagation technique of Arnold Red Honeysuckle, it would offer theory and techniques support for rapid propagation in factory.In cutting experiment , the result showed that different exogenous hormones with different concentrations had very significant on average rate of rooting、average root length and germination rate, but had not obvious effects on average root number and callus rate. The treatment of ABT1 (100 mg.kg-1) was the best for average rate of rooting and average root length ,it had the highest percentage of rooting (87%)and longest average root length(46.92mm). At the concentration of 100mg.kg-1 ABT1, the germination rate reached to highest (90%).As a whole, ABT1 had a more stable promotion on rooting of Arnold Red Honeysuckle than IBA.In drought stress experiment, the result showed that there were many changes in physiological indexes contents of Arnold Red Honeysuckle, to some degree which reflected the adjustment and adaptation of Arnold Red Honeysuckle under drought stress. The whole change trend of contents of free proline、soluble sugar、peroxides、chlorophyll、bound water were good for Arnold Red Honeysuckle adapting in drought stress; although the tissue water content downtrended, it maintained at a hight level. Integrating above indexes manifestations, the Arnold Red Honeysuckle had strong drought resistance.In salt stress experiment, the result showed that, in different soil environment with different salt (Nacl) levels, the content of MDA and Pro showed different changes.When soil salt content within 4.5g/kg, Arnold Red Honeysuckle had strong salt resistance;and it could adapt to salt stress in some extent by autoregulation.更多还原