【作者】 徐冰；

【导师】 韩烈保；

【作者基本信息】 北京林业大学 ， 草业科学， 2008， 硕士

【摘要】 为了验证并比较CMO-BADH双基因、CMO基因和DREB1A基因的抗旱和耐盐性功能,评定各转基因株系抗旱和耐盐能力,本研究通过对非转基因草地早熟禾、转CMO-BADH双基因草地早熟禾、转CMO基因草地早熟禾和转DREB1A基因草地早熟禾进行干旱胁迫试验和不同浓度的NaCl胁迫试验,测定了其叶片相对含水量、细胞膜的透性、丙二醛(MDA)含量、超氧化物歧化酶(SOD)活性、过氧化物酶(POD)活性、氧化氢酶(CAT)活性等,结果表明:CMO-BADH双基因、CMO基因和DREB1A基因的转化均能提高草地早熟禾的抗旱性和耐盐性。本研究还通过测定转基因T1代种子和非转基因商品种在PEG胁迫条件下的相对发芽率,评定了转基因T1代种子和非转基因商品种子的抗旱能力,结果表明:转基因T1代种子的抗旱能力大于非转基因商品种子的抗旱能力。上述研究为草地早熟禾抗旱耐盐新品种的选育提供了理论依据和植物材料。更多还原

【Abstract】 In this experiment, in order to analysis and compare the functions of CMO-BADH double gene、CMO gene and DREB1A gene, to order the salt tolerance and the drought tolerance of all lines and to analysis the drought tolerance ability of T1 filial generation, the effects of drought resistance and different NaCl concentration stress to the tissue culture of transgenic Kentucky bluegrass with CMO-BADH double gene、CMO gene and DREB1A gene were studied on. And the relation water content (RWC), relative electrical conductivity, MDA content, SOD activity, POD activity and CAT activity were measured. The results showed that the CMO-BADH double gene, CMO gene and DREB1A gene all could enhance the drought and salt tolerance of transgenic Kentucky bluegrass.Through studying the relative germination percentage of T1 filial generation and commercial breed under PEG drought resistance,the drought tolerance of the T1 filial generation and commercial breed was ordered. The results showed that the drought tolerance of T1 filial generation is better than commercial breed. And this study also provided theoretical evidence and plant materials for breeding the new Kentucky bluegrass varieties tolerant to drought and salt.更多还原