基于SPR技术的检测装置设计与实验研究

【作者】 曾翰蓉；

【导师】 王晓萍；

【作者基本信息】 浙江大学 ， 测试计量技术及仪器， 2008， 硕士

【摘要】 基于表面等离子体共振（Surface Plasmon Resonance,SPR）的监测仪器具有灵敏度高、检测速度快、样品消耗量少、无需标记生物样品等特点,能够广泛应用于化学、生物、环境、食品、医疗、制药等领域。但是,目前国内外大部分SPR仪器都存在结构复杂、体积庞大、成本高等缺点,限制了其在现场监测、实时分析等方面的运用。因此,研制小体积低成本的SPR检测装置,必然能够推广SPR技术在各领域的应用,产生巨大的社会效益和经济效益。本文在仔细分析SPR检测仪器应用领域、现状和发展趋势以及传感原理的基础上,自主开发了一套小型SPR检测装置。该装置主要包括SPR传感器、微流恒速进样系统、信号检测与控制电路以及计算机数据分析处理软件。传感器采用宽光束入射的非扫描式角度调制方式,简化了传统具有可动部件的角度扫描方式,简化了SPR传感器结构,减小了体积。微流恒速进样系统由高精度注射泵和智能多位阀组成,可满足极微量样品的选择以及控制样品进样速度的恒定,并可进行样品、缓冲液和再生液的自动选择进样,保证测量过程的自动完成。信号检测与控制系统和计算机数据分析处理软件为SPR仪器提供了强大的数据采集与分析处理功能,使其具有实用性强、操作方便等特点。利用自行设计的小型SPR检测装置,搭建一套基于SPR技术的在线折射率计。对不同浓度的葡萄糖溶液进行测量,结果表明该装置具有较好的性能,其折射率检测灵敏度达到5×10^-5RIU（折射率单位）,能够测量的葡萄糖溶液的最低浓度可达0.01%。利用自行设计的小型SPR检测装置,搭建一套基于SPR技术的生物传感器。简要地分析SPR生物检测原理与检测过程,在此基础上,通过磺胺甲恶唑（sulfamethoxazole,SMX）抗原浓度的实验,表明该装置在生物分子相互作用方面的直接、实时在线观测的能力。更多还原

【Abstract】 Surface plasmon resonance （ SPR ） monitor instrument has the advantage of high-sensitivity, fast detection, less sample needed and label-free analysis. It can be widely used in chemistry, biometrics, environmental monitor, food detection, clinic diagnosis, and medicine etc. However, most SPR instruments have the disadvantage of complex structure, huge physical volume and high cost. The disadvantage limits its application in field monitor and real-time analysis. As a result, developing a small and low-cost SPR monitor instrument will spread SPR technique using in each area. It will bring huge social benefits and economic benefits.This research carefully analyses the application area, developing trend and sensor principle of the SPR monitor instrument. We developed a set of small SPR monitor instrument independently. The instrument consists of a SPR sensor, a sampling system with tiny and constant flow, a signal detection and control circuit and analysis processing software. The SPR sensor adopts non-scanning modulation structure with broad beam. This modulation is based on the traditional scanning modulation with moving parts. But it reduces the physical volume of SPR sensor. The sampling system with tiny and constant flow consists of high precision pump and multi-position micro-electric valve. This system can control the speed of sample and choose different samples. All the measuring process is auto finished. The signal detection and control circuit and analysis processing software provide the function of data acquisition and analysis processing for SPR instrument.Dextrose solutions of different concentration are measured by our small SPR instrument. The result presents that the instrument has good performance. The sensitivity reaches approximately 5×10^-5RIU, the minimum concentration can be detected is 0.01%.In the paper we also analyze the SPR principle of biological detection and detection process briefly. SMX is measured by our small SPR instrument. The result presents that the system’s powerful ability on directly monitoring interactions between biomolecules.更多还原