【作者】 张帆；

【导师】 李先恩； 曲延英；

【作者基本信息】 新疆农业大学 ， 作物遗传育种， 2008， 硕士

【摘要】 粉葛是甘葛藤（Pueraria thomsonii Benth.）的干燥根,是一味常用中药。近年来,粉葛综合利用价值日益提高,已成为重要的药用植物和工业生产原料。本实验对粉葛快速繁殖体系及试管块根的诱导条件进行了研究,并探索了⁶⁰Coγ射线辐照对粉葛组培苗生长发育的影响。取得了以下结果:1、粉葛试管快繁技术的研究粉葛种子的休眠主要是种皮的不透性引起的,通过机械破坏种皮的方式能使种子的萌发率达到80%。采用75%酒精和0.1%升汞作为种子表面灭菌的灭菌剂,采用两次消毒的方法可将污染率降低到15%。试管苗的增殖培养基为:MS+6-BA2.0mg/L+NAA0.1mg/L ,增殖系数为18% ;试管苗壮苗培养基为:MS+6-BA0.1mg/L+NAA0.1mg/L;试管苗的生根培养基为:1/2MS+IBA1.0mg/L,生根率为90%。2、粉葛试管块根诱导技术的研究明确了粉葛试管块根诱导的最适培养基是:MS+6-BA1.5mg/L+NAA0.1mg/L。本研究发现较高的蔗糖含量和较高的温度能促进粉葛试管块根的增粗,最适宜的含糖量和温度分别为5%和25℃,试管块根直径可达0.6cm;添加外源GA3不利于试管块根形成。3、⁶⁰Coγ射线辐照对粉葛试管苗生长的影响随着辐照剂量的增大试管苗的生根受到抑制,在剂量率为1Gy/min的处理中,当剂量达到40Gy以上时,试管苗不再具备生根能力;当辐照剂量不超过10Gy时γ射线对试管苗的增高和增殖均有一定的促进作用,当剂量超过10Gy时,随着剂量的增大,试管苗的生长受到抑制,当剂量达到60Gy时,试管苗呈生长停滞状态;粉葛试管苗最适剂量是剂量率为1Gy/min条件下辐照计量为20Gy,致死剂量是剂量率为1Gy/min条件下辐照剂量为100Gy。更多还原

【Abstract】 Radix Puerariae, the dry root of Pueraria thomsonii Benth., is one of traditional Chinese medicines. In recent years, Radix Puerariae powder, whose comprehensive value in use has been improved gradually became significant officinal and raw material for industry production. In this study, we focused our attention on the establishment of rapid propagation system and the induction condition of the tube earthnut, and also the effect of 60Coγray irradiation on the growth of cultured seedlings of Radix Puerariae. The results are listed as follow:1. Study on the establishment of rapid propagation system of Radix Puerariae.The dormancy of Radix Puerariae.seeds is caused by the imperviousness of seed coat, which can be smashed by machinery break and reached 80% of the germination rate . Sterilized by 75% ethanol and 0.1% HgCl2 for 2 times can reduce the pollution rate to 15%. Propagation culture medium of tube seedling is MS+6-BA2.0mg/L+NAA0.1mg/L, by which the grpgation coefficient was 18%; sound seedling culture medium is MS+6-BA0.1mg/L+NAA0.1mg/L; root inducing culture medium is 1/2MS+IBA1.0mg/L, and the root gemination rate was 90%.2. Study on induction condition of the tube earthnut of Radix Puerariae.The optimum tube earthnut induction culture medium is MS+6-BA1.5mg/L+NAA0.1mg/L; high sucrose content and higher temperature were both beneficial for the increase of the tube earthnut diameter., and the most suitable sucrose content and the optimum temperature for the tube earthnut induction were 5% and 25℃respectively; exogenous GA3 went against the formation of tube earthnut.3. The impact of 60Coγray irradiation on the growth of Radix Puerariae tube seedlings.The rooting ability was inhibited as the dose ofγray increased, when the dose was up to 40Gy and with the treatment of 1Gy/min the rooting ability of tube seedlings lose; and the dose lower than 10Gyγray can increase the height and propagation of tube seedling;when the irradiation dose surpass 10Gy the growth can be inhibited, and the dose upper to 60Gy can absolutely stop the growth of tube seedling; the optimum dose for the Radix Puerariae tube seedlings is 20Gy with the treatment of 1Gy/min, the fatal dose was 100Gy with the treatment of 1Gy/min.更多还原