【作者】 孙家行；

【导师】 刘军；

【作者基本信息】 吉林大学 ， 临床医学， 2008， 硕士

【摘要】 本试验分为先接旋毛虫后荷瘤组和先荷瘤后接旋毛虫组。先接旋毛虫后荷瘤组BalB/C小鼠先接种不同剂量的旋毛虫、接种11天后荷以C6胶质瘤细胞,先荷瘤后接旋毛虫组BalB/C小鼠先荷以C6胶质瘤细胞,11天后接种不同剂量的旋毛虫。通过对试验小鼠体内肿瘤的重量指标检测、小鼠死亡率及其与未长瘤小鼠比率的分析,研究旋毛虫对小鼠体内C6胶质瘤细胞生长的抑制作用。同时测定试验小鼠机体免疫指标,探讨旋毛虫抗肿瘤机理。研究发现旋毛虫对C6胶质瘤细胞在小鼠体内的生长有一定的抑制作用,随着旋毛虫接种剂量的增加,抑制效果越明显,但同时要考虑对小鼠的伤害程度。接种600条的实验小鼠身体消瘦、被毛凌乱、食欲不振、小鼠死亡率较高。不同接种时间对C6胶质瘤细胞的抑制作用也有差异,先接旋毛虫后荷瘤组的抑瘤效果好于先荷瘤后接旋毛虫组(即接种11d后荷瘤组抑瘤效果较好)。更多还原

【Abstract】 Tumor is a kind of disease endangering human health greatly. The environment pollution from human industry makes the global malignant tumor population increase largely. Every year in China, there are about 1.6-2,000,000 new reported cases, the incidence is developing at 3%, and the age of onset is turning younger. Traditional treatments include operation, chemotherapy and radio- therapy. But it staggers the anti-tumor effect that the normal tissue cells are also damaged in the treatment process due to the lack of good targeting. Biotherapy is called the 4th management for tumor treatment following the routine 3 therapies, which makes its effect by inhibiting tumor growth from induction of human natural defense mechanism or supplying body new substance. Recent years, it attracts more attention to the study of this kind of treatment. A lot of research discovers that, besides animals and plants, there are many virus and parasites helping increase body immune system against tumor. The effects of parasites for the antineoplasmic acti- vity, by reviewing the published papers, we conclude the following 2 sides. On the one hand, the parasites activate body immune system to enhance the antineoplasmic activity; on the other hand, the active substance, excreted from parasites, kills tumor cells directly, actives tumor apoptosis, and induces immune system against tumor.Trichinelliasis, caused by trichina cystica infection, is a kind of cosmopolitan zoonosis, but some magic results of the infection, reported by some researchers, involve the antineoplasmic activity. In labs, the rat after trichina cystica infection, gains the ability of antineoplasmic activity, or rejects the transplanted tumor cells, but it reacts differently to different tumor cells. With the advancement of molecular biologic technology, more and further research and investigation are carried out. Though neoplastic cells and parasites are different originally, the neoplasm has its own metabolism and the parasite has its own infection way, on the molecular level, they have something in common, altering AAG to avoid immune attack, blocking AAG, destroying the immune monitoring, and so on. Due to the molecular similarity, body immune system rejects tumor at the same time of its defense against parasites infection. Unfor- tunately, till now, little is known about the total nature of the antineoplastic ability of parasites and no report is published on the vaccination from it.In our study, we divide rats into the prevention group and the treatment group, for the aim to observe the antineoplasmic activity from trichina cystica infection, by determining the tumor mass size, and the comparison of rat mortality. And at the same time, we discuss the mechanism by analysis of the immune index. The research results turn out that, trichina cystica infection inhibits the growth of C6 glioma transplanted in rats’body, and the inhibition effect is dosage positive percentage to trichina cystica infection, while the destroying effect of it must be taken into consideration. The rats, inoculated with 600 parasites, present loss of appetite, tabid, hair disorder and , high mortality. It makes great difference, at what time the infection is administrated. In our study, the prevention group reacts better than the treatment group. After the infection of larva into host, the larva develops and makes its lifecycle in the host body, and the host makes specific immunologic response to the infection. In the 11th day after inoculation, when it’s the migratory stage of larva, the larva moves from blood to the body muscle tissue, causes immune system respond greater, and in consequences the antineoplasmic activity is enhanced. In the detection of T cell subgroup, we conclude that the level of CD3+ and CD4+/CD8+ increases after infection, and the increasing level is related to the duration and the dosage of the inoculation. In some sense, our research confirms the inhibition effect of Trichina cystica for glioma and makes further discussion.更多还原