【作者】 谌科；

【导师】 叶章群； 胡志全；

【作者基本信息】 华中科技大学 ， 外科学， 2006， 硕士

【摘要】 目的研究姜黄素和顺铂联用抑制雄激素非依赖性前列腺癌细胞株PC-3的增殖及诱导凋亡的作用。方法不同浓度的姜黄素(10μmol/L、25μmol/L、50μmol/L、75μmol/L、100μmol/L)和顺铂(3 mg/L)分别和联合作用于雄激素非依赖性前列腺癌细胞株PC-3,MTT比色法检测细胞生长活性,透射电镜观察细胞超微结构变化。流式细胞仪测定细胞周期时相变化及检测细胞凋亡。结果姜黄素显著抑制PC-3细胞的生长,呈剂量和时间依赖性。不同浓度姜黄素组之间与不同作用时间组之间的差异均有显著性意义(P均<0.01);顺铂联合不同浓度姜黄素可显著抑制PC-3细胞增殖(P均<0.05),差异有显著性意义。50μmol/L姜黄素和3 mg/L顺铂联合作用PC-3细胞24h后,细胞出现凋亡形态学改变。各浓度姜黄素组作用PC-3细胞24h后,凋亡细胞比例均显著高于空白对照组(P均<0.05),差异有显著性意义, PC-3细胞出现剂量依赖性G2/M期阻滞(P<0.01),姜黄素及顺铂均可诱导PC-3细胞凋亡。两种药物联用后,诱导细胞凋亡作用增强。结论姜黄素通过诱导前列腺癌细胞株PC-3的凋亡从而抑制肿瘤细胞的生长。姜黄素与顺铂能够协同抑制人前列腺癌细胞株PC-3的增殖,两种药物联用有增效的作用。更多还原

【Abstract】 Objective: To study the growth inhibition effects of Curcumin and Cisplatin on androgen independent prostate cancer cell line PC-3.Methods: The growth activities of cancer cells were studied by MTT colorimetry. Cell cycle phases and the apoptosis were inspected by flowcy-tometery (FCM). The morphological changes of cancer cells were observed under electronic microscopy.Results: Curcumin obviously suppressed the proliferation of PC-3 cell line in does-dependent and time-dependent manners in vitro. There was an obvious difference of inhibition rate in different concentrations and time groups (P<0.05). Curcumin could lead to characteristic morphological changes of apoptosis in PC-3 cell line after 24 hours. The ratios of apoptosis cell were significantly higher than that of controls (P<0.05) after curcumin influence the PC-3 cell line. Curcumin could arrest the cell cycle of PC-3 cell line at G2/M phase in does-dependent manners (P<0.01). Thses effects could enhance significantly by cisplatin. The curcumin and cisplatin can all induce cell line PC-3 apoptosis. When the two drugs combined, the inductive effect for cell apoptosis became obvious.Conclusion: Curcumine could suppress the growth of androgen-indenpent prostate cancer cell lines and promote their apoptosis by different mechanism. Cisplatin can enhance antiproliferative effect of curcumin to hunman prostate cancer cell line PC-3.更多还原