【作者】 毕玉霞；

【导师】 韦旭斌；

【作者基本信息】 吉林大学 ， 临床兽医， 2007， 硕士

【摘要】 大量研究表明,许多中草药具有抗病毒、抗炎、提高机体免疫力等作用,为此,我们以中兽医药学理论为指导,设计了中药制剂参灵清瘟败毒口服液,以针对鸡传染性支气管炎病的病因,提高鸡传染性支气管炎(infectiousbronchitis)的治疗效果,其主要配方为人参、灵芝、板蓝根、黄芪、栀子等。本文通过鸡传染性支气管炎病毒M41株感染鸡胚试验,观察了参灵清瘟败毒口服液抗传染性支气管炎病毒M41株鸡胚感染的抑制作用;应用参灵清瘟败毒口服液对人工感染和自然感染鸡传染性支气管炎的疗效试验,判定该药对鸡传染性支气管炎的疗效。结果表明,参灵清瘟败毒口服液对胚胎的发育无任何不良影响,不同浓度的药液对传染性支气管炎病毒感染鸡胚均有保护作用;参灵清瘟败毒口服液对人工感染和自然感染的鸡传染性支气管炎都有很高的治愈率,均高于82%。保护效果也很好。综上所述,参灵清瘟败毒口服液对鸡传染性支气管炎具有良好的防治效果,并且具有明显抗传染性支气管炎病毒的作用。更多还原

【Abstract】 In order to improve the curative effect on infectious bronchitis in chickens, we designed a chinese medicinal prescription-“SLQWY”according to the pathogenesis. This prescription mainly consists of ginseng, Ganoderma lucidum, isatidis,radix, Astragalus mongholicus, Gardenia augusta Merr. To investigate its effect, we carried out this study from three aspects.1 The effect on infectious bronchitis virus M41 in embryonated eggs of SLQWYWith the embryonated eggs infected with the standard M41 strain of IBV, the inhibition effect of SLQWY were investigated. The results showed that SLQWY has no any side effect on the development of the embryos, and different concentrations selected can protect the embryos, indicating that SLQWY has marked antiviral effect.2 Preventive and curative effect of SLQWY on the chickens experimentally artificial infected with infectious bronchitis virus M41Seven hundred 3-week-old Hy-line variety brown chickens were experimentally infected with the standard M41 strain of IBV,and then divided into seven groups at random. Before and after inoculation, different dosages of SLQWY were added into drinking water to observe the incidence rate, clinical symptom, fatality rate, and pathological alterations, with moroxydine as positive control drug. The results showed that SLQWY can protect the infected chickens. Nought point six seven permillage of SLQWY in drinking water can effectively cure the chickens experimentally infected with infectious bronchitis virus, and the curative rate is 86.25%, higher than that of moroxydine treated group.3 Curative effect of SLQWY on the chickens naturally infected with infectious bronchitis virus M41Through epidemiological survey, clinical symptom identification, pathological alterations, and laboratory examination, 4827 chickens were diagnosed to be naturally infected with infectious bronchitis virus, and then divided into several groups at random. Different dosages of SLQWY were added into drinking water to observe fatality rate, clinical symptom, and curative rate. The results showed that 0.67‰of SLQWY can effectively cure the chickens naturally infected with infectious bronchitis virus, and the curative rate was 92.02%. Nought point six seven permillage added into drinking water is recommended to be the therapeutic dose to treat the infectious bronchitis.These data indicated that SLQWY has favorable preventive and curative effect on infectious bronchitis in chickens and marked antiviral effect.更多还原