【作者】 宋建；

【导师】 吴晓梅；

【作者基本信息】 四川大学 ， 眼科学， 2007， 硕士

【摘要】 目的：研究睑板腺癌SSTR2的表达情况，探讨SSTR2表达与睑板腺癌病理学特点之间的关系。方法：收集睑板腺癌组织标本60例及正常睑板腺组织标本20例。每个标本制成5μm厚组织切片3张，一张行HE染色用于病理学分类，一张采用免疫组化法检测SSTR2的表达，一张以PBS液代替一抗作为空白对照。采集图像，利用IPP(Image—Pro Plus 6.0)分析图象，通过测图像MOD(Mean Optical Density平均光密度)值判断图片染色强度。采用SPSS13.0统计学软件进行分析比较SSTR2的表达与睑板腺癌病理学特征间的关系及正常组织组与睑板腺癌组织间的差异性。结果：正常睑板腺组织及睑板腺癌组织中均有SSTR2的表达且均表达于细胞浆膜上。睑板腺癌组织MOD值明显大于正常睑板腺组织，差异有统计学意义(p=0.000＜0.05)。睑板腺癌不同分化程度组间MOD值存在差异，但差异无统计学意义(p=0.898＞0.05)。睑板腺癌SSTR2的表达与患者的年龄、性别、发病部位、病程、肿瘤分化程度、复发情况、转移情况等病理学特点之间存在差异，但差异无统计学意义(p均＞0.05)。结论：正常睑板腺组织及睑板腺癌组织细胞浆膜上均有SSTR2的表达。睑板腺癌组织中SSTR2表达高于正常睑板腺组织，SSTR2的表达与睑板腺癌的分化程度无明显相关。更多还原

【Abstract】 Purpose To detect the expression of somatostatin receptor 2 (SSTR2) ofthe specimens of meibomian gland carcinoma. To discuss the relationshipbetween the expression of SSTR2 and pathologic characteristics of thetumor.Methods 60 specimens of meibomian gland carcinoma and 20specimens of normal meibomian gland were collected. Every specimen wasmade 3 sections, thickness of the section was 5μm. One section was stainedby HE; one section was stained by Streptavidin-Peroxidaseimmunohistochemistry for the expression of SSTR2; one section was used forblank control by using PBS substitution the first antibody. Pictures wereanalyzed by Image—Pro Plus 6.0 (IPP 6.0). The degree of stain wasdetermined by Mean Optical Density (MOD) of sections. The difference inexpression of SSTR2 between carcinoma of meibomian gland tissues andnormal meibomian gland tissues was analysed by SPSS, version 13.0. Results Expression of SSTR2 was positive both in normal meibomiangland tissues and carcinoma of meibomian gland. The distribution of SSTR2was in plasma and membrane. MOD values in carcinoma of meibomiangland tissues were higher than it in normal meibomian gland tissues. Thedifference of MOD values was significant statistical (p=0.000＜0.05). MODvalues were different in different differentiation of carcinoma of meibomiangland tissues, but the difference was not significant statistical (p=0.898＞0.05). We found no significant statistical relationship between theexpression of SSTR2 and age, sex, position, course of disease,differentiation, recurrence, metastasis (p＞0.05).Conclusions Expression of SSTR2 was positive both in normalmeibomian gland tissues and carcinoma of meibomian gland. Thedistribution of SSTR2 was in plasma and membrane. Expression of SSTR2in carcinoma of meibomian gland tissues was higher than expression ofSSTR2 in normal meibomian gland tissues. In expression of SSTR2, wefound no relationship in differentiated degree of meibomian gland carcinoma.更多还原