【作者】 黄剑；

【导师】 卓廉士；

【作者基本信息】 重庆医科大学 ， 针灸推拿学， 2007， 硕士

【摘要】 目的:观察电针对膝关节骨性关节炎家兔模型关节冲洗液中细胞因子IL-1β和TNF-a的影响。方法:将新西兰大白兔40只随机分为空白对照组(A组)、模型对照组(B组)、手法治疗组(C组)、电针治疗组(D组)。除A组外,其余三组将大白兔左膝关节伸直位石膏固定7周制成骨性关节炎动物模型,造模成功后,A组和B组正常饲养,不处理;C组给予推拿按摩加功能训练,每天先按、揉、摩、擦患侧膝关节,再屈伸、旋转患侧膝关节,每次15分钟,最后平地跑动100米。D组取左后肢“阳陵泉、血海、足三里和梁丘”四个穴位,电针治疗,电压6V,疏密波型,频率1.6～2Hz,输出调节强度1～2毫安,以局部皮肤肌肉轻微颤动为度,每天一次,每次电针两组,每次留针25分钟,连续治疗21天。处理前、后分别取各组家兔关节冲洗液,采用放射免疫分析法(RIA),测定关节冲洗液中IL-1β和TNF-a的含量。结果:①造模动物左侧膝关节伸直位石膏固定7周后,从A、B、C、D组中各随机抽出1只动物作病理切片观察,解剖A组动物,肉眼观察膝关节面光滑呈淡蓝色,B、C、D组动物关节软骨面粗糙呈黄色,边缘骨质破坏,有骨赘增生。病理切片HE染色后,观察发现A组动物关节滑膜无增厚,软骨细胞排列均匀,无增生,软骨边缘光滑,B、C、D组动物关节滑膜增厚,软骨细胞排列不均,边缘破坏,粗糙不平,有软骨细胞增生。②处理前,B、C、D组动物关节冲洗液中IL-1β和TNF-a的含量与A组比较有显著性差异(P<0.05),B、C、D组组间比较无显著性差异(P>0.05)。③C、D组动物治疗前后比较,关节冲洗液中IL-1β和TNF-a的含量有显著性差异(P<0.05)。④处理后,D组动物关节冲洗液中IL-1β和TNF-a的含量与B组比较有显著性差异(P<0.05)。结论:电针治疗能降低膝骨性关节炎家兔模型关节冲洗液中细胞因子IL-1β和TNF-a的含量,从而发挥对膝骨性关节炎的防治作用。更多还原

【Abstract】 Objective:To observe the effects of Electroacupuncture on cell factors IL-1βand TNF-a in the Rabbit models with joint liquid of Knee Osteoarthritis.Methods:The New Zealand rabbits were randomly divided into the control group(A),the model group(B),the Massage group(C) and the Electroacupuncture group(D).The rabbits in group B,group C and group D were made into the models with the Knee Osteoarthritis. Group A and B were not treated. Group C were treated by Massage and functional training. Group D were treated by Electroacupuncture.Then the observation was conducted on the changes of IL-1βand TNF-a in the Knee Joint Liquid of these groups and on the curative effects of Electroacupuncture.Results:After Electroacupuncture was given for 3 weeks,the contents of IL-1βand TNF- a were lowered distinctively.Compared to the group D and B, the differences were obvious( P<0.05). Compared to front treatment of the group D, the differences were obvious( P<0.05).Conclusions:The Electroacupuncture has the preventive and curative effects on Osteoarthritis by lowering IL-1βand TNF-a in the Knee Joint Liquid.Acupuncture and Moxibustion for Knee Osteoarthritis is effective.更多还原