【作者】 石敦义；

【导师】 张桂蓉；

【作者基本信息】 重庆医科大学 ， 内科学， 2007， 硕士

【摘要】 目的:建立哮喘动物模型,探讨灵芝孢子对哮喘豚鼠肥大细胞激活机制的调控作用。方法:10%卵蛋白腹腔注射致敏后雾化吸入1%卵蛋白诱导建立哮喘动物模型。将30只健康豚鼠按随机数字表分为生理盐水对照组(A),哮喘模型组(B),灵芝孢子组(C)三组,每组10只,分别用生理盐水,灵芝孢子对各组豚鼠进行灌胃干预治疗15天。测定哮喘豚鼠的引喘潜伏期,肺泡灌洗液计数白细胞总数及分类,肺组织H-E染色和免疫组化观察肺组织病理变化及肥大细胞类胰蛋白酶分布情况。结果: 1、灵芝孢子组哮喘豚鼠的引喘潜伏期明显较哮喘模型组延长。2、哮喘组肺泡灌洗液白细胞总数及嗜酸性粒细胞比例较生理盐水组增高,肺组织炎性病变明显;灵芝组肺泡灌洗液白细胞总数、嗜酸性粒细胞比例及肺组织炎性病变明显较哮喘组降低或减轻。3、哮喘组肥大细胞类胰蛋白酶染色阳性的细胞计数明显较生理盐水组增多,主要分布在气道粘膜下,肺泡间隔及血管周围;灵芝孢子组类胰蛋白酶染色阳性的肥大细胞计数较哮喘组显著减少。结论:1、单纯卵蛋白致敏和雾化能够成功复制出哮喘动物模型。2、灵芝孢子有延长哮喘豚鼠引喘潜伏期,减轻肺组织炎性病变,抑制肥大细胞激活释放类胰蛋白酶的作用。更多还原

【Abstract】 Objective: To establish asthma animal model and investigate the effect of Ganoderma spores on mast cell in asthmatic guinen pigs .Methods: The asthmatic guinea pigs model was set up by intraperitoneal injection of 10% OVA(ovalbumin) for sensitizing and exposure to aerosolize 1% OVA for inducing asthma. 30 guinea pigs were divided into 3 groups by random number meter : a NS group(A), an asthma group(B) and a ganoderma spores group(C),with 10 guinea pigs in each. NS or ganoderma spores were infused into stomach of guinen pigs for fifteen days. The incubation period of asthma was detected , and the total cell numbers and classification in bronchoalveola lavage fluid(BALF) were studied .The lung tissues were stained with Hematoxylin-Eosin(H-E) to observe the pathological changes and with anti-human tryptase antibody(AAI) by using immunohistochemical technique to count the mast cell tryptase (MCT) .Results:(1) Compared with asthma group, ganoderma spores could markedly prolong the incubation period of asthma,and the mast cell tryptases counts in ganoderma spores group decreased significantly.Meanwhile, the total cell numbers and the percentage of eosinophil of BALF in ganoderma spores group were lower than those in asthma group and the inflammation response in lung tissue were significantly slighter. (2) Compared with NS group, asthma group had more leukocytes,higher percentage of eosinophil in BALF and more mast cell tryptase expression in airway submucosa, alveolar walls and vascular surrounding.The inflammation response in lung tissue of asthma group was evident.conclusion:(1)Intraperitoneal injection and atomization of OVA could duplicate asthma animal model successfully.(2) Ganoderma spores could prolong the incubation period of asthma , obviously relieve inflammation response in airway and lung tissue,and also depress mast cell activation.更多还原