【作者】 卢东红；

【导师】 唐星火；

【作者基本信息】 广西医科大学 ， 消化内科， 2007， 硕士

【摘要】 目的:研究T淋巴细胞亚群在乙肝相关疾病患者外周血中的变化及其临床意义,并探讨乙肝相关疾病血中乙肝病毒脱氧核糖核酸(HBV-DNA)载量与T细胞免疫的关系。方法:应用流式细胞仪检测乙肝病毒携带者(ASC)21例、慢性乙型肝炎(CHB)26例、乙肝肝硬化(LC)患者30例、原发性肝癌(PHC)31例及20例健康人的外周血T淋巴细胞亚群,采用荧光定量聚合酶链反应(FQ-PCR)检测上述108例乙肝相关疾病患者血清的HBVDNA的含量,并进行统计分析。结果:与对照组相比较,ASC、CHB组及LC组的CD3~+(P＜0.05)、CD4~+(P＜0.05)、CD4~+/CD8~+显著性下降(P＜0.01);PHC组CD4~+、CD4~+/CD8~+比值有显著性上升(P＜0.01),CD8+无统计学差异。对照组、HBV-DNA阳性组和HBV-DNA阴性组三组比较,CD3~+、CD4~+、CD8~+、CD4~+/CD8~+项指标均有显著性差异(P＜0.01)。HBV-DNA载量与CD3~+、CD4~+、CD4~+/CD8~+均有负相关关系,HBV-DNA的载量与CD8~+有正相关关系。结论:乙肝相关疾病患者细胞免疫功能紊乱,外周血T淋巴细胞亚群发生改变与HBV-DNA载量有关系。更多还原

【Abstract】 Objective:To study the level of T-lymphocyte subsets and its significance in the peripheral blood of patients with hepatitis B virus-related diseases,and to investigate the correlation between T-lymphocyte subsets and HBV-DNA loads.Methods:The level of T-lymphocyte subsets were detected by flow cytometry, when HBV-DNA loads were detected by FQ-PCR in 108 cases of peripheral blood,including 21 cases of ASC,26 of CHB,30 of LC,31 of PHC and 20 of healthy people.Results:The level of CD3~+,CD4~+,CD4~+/CD8~+ inASC,CHB,LC,PHC groups were significantly lower than that in control group(P＜0.05,P＜0.05, P＜0.01,respectively),when the level of CD8~+ in ASC,CHB,LC,PHC groups and the level of CD4~+、CD4~+/CD8~+ in PHC group were significantly higher than that in control group,without any significant difference between the level of CD8~+ in PHC and control groups.The level of CD3~+,CD4~+,CD8~+,CD4~+/CD8~+were all significantly different between HBV-DNA positive and negative groups. HBV-DNA loads had negative relationship with CD3~+,CD4~+,CD4~+/CD8~+ and had positive relationship with CD8~+.Conclusions:The cell-mediated immunity in patient with hepatitis B virus-related diseases was disordered,and the HBV-DNA loads are involved in the progress of these diseases.更多还原