【作者】 成先桂；

【导师】 梁伶；

【作者基本信息】 广西医科大学 ， 皮肤病与性病学， 2007， 硕士

【摘要】 目的对不同来源的马尔尼菲青霉菌(Penicillium marneffei,PM)菌株的致病过程和致病力情况进行初步探讨。方法分别将来源于广西银星竹鼠的PM菌株(野生菌株)及广西地区PSM患者的血液培养分离的PM菌株(临床分离菌株)的菌悬液经腹腔注射感染健康小鼠,然后按时间分批处死,肉眼观察并分析脏器感染情况;取其内脏(肝、脾、肺、心、肾、肠、脑、肌肉、胸骨、皮肤)及颈动脉血行真菌培养并比较阳性率差异;分析死亡小鼠的一般状况;主要脏器行组织病理切片观察了解其病理改变。结果发现小鼠感染的发病率为100%;不同时期小鼠内脏病变程度有差异,且内脏病变程度随着时间推移而逐渐减轻;A、B两组小鼠部分脏器真菌培养阳性率有明显差异;A、B两组小鼠的死亡率无显著性差异,死亡小鼠存活时间的中位数为24.42天;A、B两组小鼠肝、脾、肺、肠、肾等脏器的基本病理形态有改变,C组脏器无病理改变。结论不同来源的PM菌株经腹腔注射感染途径均可使小鼠致病;说明竹鼠体内野生PM与人类马尔尼菲青霉病(Penicilliosis marneffei,PSM)关系密切;具有正常免疫功能的小鼠感染PM后可以逐渐恢复;本实验可以作为研究马尔尼菲青霉病的发病机制、病理学及致病力等的一个手段。更多还原

【Abstract】 Objective To study the pathopoiesis and process of wild isolates and clinical isolates penicillium mameffei on mice through abdominal cavity injection.Methods The conidia suspension of wild isolates and clinical isolates penicillium mameffei was inoculated into the abdominal cavity of healthy mice respectively.We killed the mice in batch on time,to observe the infection status of internal organs and masculine rate of PM infections,to analyze general state for death mice,and to study histopathological microscopical diversity for main internal organs.Result The incidence of mice infected with penicillium marneffei was 100%;The affection of splanchna was diverse in five periods,and it was less and less prominent with time-lapse.The masculine rate of PM infections was different for some different organs between group A and group B.The mortality rate was identical between group A and group B,and the median of survival time was 24.42 days for death mice;The histopathological microscopical diversity was evident for main internal organs between group A and group B, and there was no change in group C.Conclusion Penicillium mameffei of different source is pathogenic on mice through abdominal cavity injection;It indicates that the relationship of wild isolates PM and Penicilliosis mameffei in people is intimate;Infection could recover spontaneously by degrees in immunocompetent mice;This experimental study might be suitable to study pathogenesis,pathology and virulence of penicilliosis marneffei.更多还原