【作者】 祁鹏志；

【导师】 姜玲；

【作者基本信息】 华中农业大学 ， 植物学， 2007， 硕士

【摘要】 本实验采集带有典型衰退病症状的江西新余蜜柑母株茎尖进行了茎尖微芽嫁接(STG)，获得了成活苗169株；并对稻叶蜜柑、市文蜜柑、翡翠柚、处红柚、瓯柑2003年以来已获得的186株STG苗及母株进行了黄龙病和衰退病分子检测；对不同浓度病毒唑对茎尖微芽嫁接成活率的影响进行了研究，主要的结果如下：1．以带典型黄龙病症状并经分子检测的柑橘作正对照，对即将出圃的1-3年龄STG苗186株进行了PCR检测，被检测样本均没有检测到黄龙病病原，无毒率100％。2．对瓯柑、稻叶蜜柑、市文蜜柑、翡翠柚、处红柚等6个品种的母株和茎尖嫁接苗进行了RT-PCR检测，结果所有样品的母株均扩增出了衰退病特异性条带，而STG嫁接成活苗的无毒率最高仅为61.1％，证明茎尖嫁接方法并不能完全脱除衰退病病原。3．对比了不同浓度病毒唑对嫁接成活率的影响，初步筛选出了适宜嫁接的最适浓度为20mg／L。更多还原

【Abstract】 In this study, the shoot-tips of Citrus sutsuma Marc from Xinyu city of Jingxi province were used as explants to perform shoot-tip grafting (STG) , 169 STG plantlets were obtained; all the mother plants of Citrus sutsuma Marc cv Daoye、Citrus sutsuma Marc cv Shiwen、Citrus grandis osback cv Feicui、Citrus grandis osback cv Chuhong、Citrus Suavissima Hort ex Tanaka and 186 STG plants ever since 2003 have been determinated with PCR and RT-PCR. We evaluated the existence of pathogery of BLO and CTV. In addition, we studied the effect of Virazole treatment on the shoot-tip grafting survival rate, the main results are as following:1. 186 STG plants of 1-3 years old were identified by PCR technique, BLO pathogen was not found, the virus-free rate was 100%.2. We detected the five varieties citrus mother plants and STG plants with RT-PCR, as a result, all of the mother plants could amplified Tristeza bands, but the highest virus-free rate of grafting plants only was 61.1%, and the results may prove that not all shoot-tip grafting can remove the CTV completely.3. Contrasted the effect of different concentration virazole on grafting survival rate, and the optimum of viratole is 20mg/L.更多还原