【作者】 邹锋；

【导师】 肖兵南；

【作者基本信息】 湖南农业大学 ， 临床兽医学， 2007， 硕士

【摘要】 家蝇在我国分布广，资源丰富，容易养殖，成本低，在新饲料资源开发及医药开发方面是一个很好的资源。为了建立了较为稳定的畜禽粪便养殖蝇蛆的方法，本文对生产蝇蛆的生态条件进行优选，得到结论：对蝇蛆生长影响最大的因素是食料，其次是温度，再次是饲养的湿度。最适培养料为麸皮、鸡粪和猪粪(1:1:1)；最适温度为25℃；最佳湿度为70％。进行家蝇抗菌肽生产工艺的优化——诱导家蝇幼虫高效表达抗菌肽，确定最佳的提取条件；并对家蝇抗菌肽的生产工艺进行简化，以适应工业化生产的要求。针对得到的抗菌肽进行生物学性质研究，包括对临床多重耐药菌的抑菌活性测定，测定抗菌肽对临床多重耐药菌的最低抑菌浓度(MIC)和最低杀菌浓度(MBC)；考察抗菌肽对温度、pH值的稳定性；进行制剂的研究，通过正交试验确定家蝇抗菌肽凝胶剂的最佳配方：家蝇抗菌肽，25g；基质(羧甲基纤维素钠)，3g；表面活性剂(二甲基亚砜)，0.1g：保湿剂(甘油)，20g；添加蒸馏水100g；pH，4.5。并进行含量测定及其对临床多重耐药菌的抑菌活性；通过离心法和高温加速法检测制剂稳定性，为今后的临床应用提供资料。最后，研究家蝇抗菌肽凝胶剂的临床疗效，探讨其临床适应症，用家蝇抗菌肽凝胶剂对临床型乳腺炎和隐性乳腺炎进行治疗，有效率分别达85％和94％，治愈率分别达60％和90％。更多还原

【Abstract】 Musca Domestic and Larvae is a good resources of feed resources and medicineresources because of its broad distribution, easy cultivation and lowcost.We started our work in order to find new active antimicrobial peptides. Weestablishing cultivation method for Musca domestica and larvae, finding a newfeeding method for musca domestica with annimal manure. Effects of environmentalfactors on mass reproduction of Musca Domestic Larvae was made in laboratory, theresults showed that the optimum temperature of adult oviposition and larvae growthwas 25℃. The optimum water content of larva diet was 70%. The factors affectinglarva weight were diet, temperature and breeding density in descending order.And then We optimize the antibacterial prptides produced technics. We detect itcan inhibit the resistant-bacterial growth, the MIC and MBC of the anmicrobialpeptide from the Musca domestic larvae was determined, and they can inhibit thegrowth of different species of multi-drug resistant Gram-positive and multi-drugresistant Gram-negative bacteria to different extent. Finally, we determine the effect ofpH and temperature on stability of antibacterial peptide.By the orthogonal design L16(4~5), we determined the prescriptions ofantimicrobial peptide gel: antibacterial peptide (25g); bases (3g); surface-activeagents (0.1g); humectant (20g); water (100g); the optimum pH was 4.5.The inhibitition of antimicrobial peptide spray and gel to resistant bacteria aremeansured. We conclude the spray and gel are potential to clinic use.Finally, the clinical efficacy and the clinical indications of Musca Domesticantimicrobial peptide gel were observed, resulting that the average cure and efficientpercentage of this antibactria peptide gel for severe clinical mastitis were 60% and85%; and the average cure and efficient percentage of 3 days-administrated this gelfor subclinical mastitis were 74% and 88%, and 90% and 94% for 7days-administrated.更多还原