【作者】 庄耘；

【导师】 沈云志；

【作者基本信息】 苏州大学 ， 消化内科， 2007， 硕士

【摘要】 目的:探讨幽门螺杆菌(HP)与胆结石形成的相关性,寻找HP在胆汁中存在的证据,同时对胆汁中细菌检测方法进行研究分析。方法:选择36例胆结石患者,其中28例采用胆管穿刺、8例采用内镜下选择性胆管插管抽取胆汁,用酶联免疫吸附法(ELISA)定量检测胆汁中HP-CagAIgG,同时与对照组(非结石组)16例进行比较,对照组中8例采用胆管穿刺、8例采用内镜下选择性胆管插管抽取胆汁。结果:结石组与对照组胆管内胆汁中HP-CagA IgG检出阳性率有显著性差异(P<0.05),伴有和不伴有急性胆管炎的胆结石患者胆管内胆汁中HP-CagA IgG检出阳性率亦有显著性差异(P<0.05),而术中胆管穿刺与内镜下选择性胆管插管抽取的胆汁中HP-CagA IgG检出阳性率无显著性差异(P>0.05)。结论:HP可能存在于胆道,并与胆结石的形成有一定的关系;经内镜下选择性胆管插管抽取胆汁进行HP-CagAIgG的检测是可行的。更多还原

【Abstract】 Objective:To study the relationship between the Helicobacter pylori infection and the formation of gallstones. To collect the evidence of HP existing in biliary tract, and to study the detective methods of bacteria in biliary tract.Methods:Twenty-eight cases of patients with gallstone collected bile by direct puncture during operation and other eight cases with gallstone by ERCP operation. HP CagA immunoglobulin G detected by enzyme - linked immunosorbent assays(ELISA). Sixteen cases of patients without gallstone was the control group. Eight cases collected bile by direct puncture during operation and other eight cases by ERCP operation.Result:The detection of HP-CagA IgG of the two groups has significant difference. There was also significant difference with/without acute cholangitis in biliary tract of the patients with gallstone. However, there was no significant difference between the direct puncture group and ERCP group.Conclusions: HP exists possibly in biliary tract and has some relationship with the formation of gallstone. It’s feasible to detect Helicobacter pylori CagAIgG through ERCP.更多还原