【作者】 宫存杞；

【导师】 谢建新； 陈文；

【作者基本信息】 石河子大学 ， 生物化学与分子生物学， 2007， 硕士

【摘要】 目的:新疆党参(Codonopsis Clematidea Clarke)为桔梗科党参属多年生草本植物,在全疆均有分布,且储量丰富,但其利用却很少。本课题期望通过对新疆党参多糖(Codonopsis Clematidea Clarke polysaccharide ,CCP)理化性质、抗肿瘤作用等的研究,探讨CCP作为一种抗癌新药的临床应用前景,为开发新的抗癌药物提供实验依据。方法:(1)用水提醇沉法从生药新疆党参中得到粗多糖,Sevag法反复萃取5次去除多糖中混合的蛋白质,用丙酮、乙醚脱水洗涤4次,冷冻干燥后透析袋透析得多糖。多糖经过Sephadex G-75凝胶柱纯化,得到较纯的的多糖样品。经过溶解性、Molish试验、Fehling试验等实验的鉴定,确定所得到成分的为多糖;采用苯酚-硫酸比色法检测多糖的含量。(2)采用小鼠肉瘤S180移植模型,对CCP的体内抑瘤率、荷瘤小鼠的生命延长率进行测定。通过脾脏指数、以及ELISA法检测血清TNF-α水平,观察CCP对免疫系统的影响。(3)从昆明小鼠腹腔分离巨噬细胞,进行原代培养,检测CCP对巨噬细胞吞噬活性的影响。结果:(1) CCP样品中多糖含量为36.01 %。(2)新疆党参多糖对荷瘤小鼠的抑瘤率为54%,生命延长率为17.7%,并且明显增加荷瘤小鼠的脾脏指数,升高血清TNF-α水平。(3) CCP在浓度30 mg/mL、80 mg/ mL、128 mg/mL均可以明显增强巨噬细胞的吞噬活性。结论:(1)CCP样品中多糖含量为36.01 %,新疆党参中多糖含量很高,具有很好的开发前景。(2)CCP具有一定的抗肿瘤作用,且与化疗药物合用具有增效作用。(3)CCP可以增加荷瘤小鼠的脾脏指数,升高荷瘤小鼠血清TNF-α水平,增强巨噬细胞的吞噬活性,说明调节机体的免疫功能可能是抗肿瘤作用的机理之一。(4)CCP在治疗癌性恶病质方面具有研究价值。更多还原

【Abstract】 Object: Codonopsis Clematidea Clarke in Xinjiang is the platicodon grandiflorum branch codonopsis pilosula.. It is distributed in Xinjiang province, and the reserves are abundant, In order to offer the basis for developing and utilizing of Codonopsis Clematidea Clarke. We did research about the medicine’s physicochemical properties and anti-tumor activity .Methods:(1)The polysaccharide was isolated by extraction with hot water and precipated with alcohol. In order to remove the mixed protein, the Sevag extract for 5 times. Polysaccharide had been washed 4 times with acetone and ether dehydration, after freeze-drying and dialysis. We got pure polysaccharide sample after Sephadex G-75 gelatin column purification. With absorbance experiment,Molish experiment and Fehling experiment , etc,. The sample we got whose ingredient is the polysacch- aride. We got the polysaccharide content through the phenol - sulfuric acid color method;(2) The anti-tumor effects of CCP were tested in mice with transplanted tumorsarcoma-180,the tumor inhibition rate, life span , speen index and level of serm TNF-αare determined.(3)peritoneal macrophage were isolated from KM mice, phagocytosis of macrophage was determined in the concentration of 30 mg/m L,80 mg/ m L,128 mg/m L.Result:(1) the CCP content is 36.01 %., (2) the tumor inhibitory rate of CCP to S180 was 54%,life prolong e rate is 17.7%,The spleen index and level of serm TNF-αin the groups treated with CCP were higher than control group(3)CCP can enhance the phagocytosis activity of macrophage greatly in the concentration of 30 mg/mL、80 mg/ m L、128 mg/m L.Conclusion: (1)the CCP content is high which will have very good prospect.(2)CCP has effective anti-tumor activity and can enhance the anti-tumor effect of CTX(3) The anti-tumor role of CCP can be induced by ehancing immune function.(4)CCP is worth to continue to be researched in cancer cachexia.更多还原