【作者】 郭燕；

【导师】 马书尚；

【作者基本信息】 西北农林科技大学 ， 植物学， 2007， 硕士

【摘要】 本论文以‘粉红女士’苹果为试材,研究了不同采收期对果实低温贮藏特性的影响,以及0℃贮藏期间和随后14d的货架期间,0.5μL·L-1浓度1-MCP(1-甲基环丙烯)对果实呼吸速率、乙烯释放速率、贮藏品质和可溶性蛋白质变化的影响,探讨了1-MCP影响苹果衰老的生理生化机制。试验结果表明:(1)采收成熟度影响低温贮藏期间果实品质,特别是贮藏期超过3个月的果实。采收成熟度越高的果实,经长期贮藏后硬度、可滴定酸含量和可溶性固型物损失越大。因此,长期贮藏的果实宜在10月中旬采收,短期贮藏的果实宜在10月末或11月初采收。(2)用0.5μL·L-1的1-MCP在20℃下处理‘粉红女士’苹果24h ,显著降低0℃贮藏期间果实呼吸速率、乙烯释放速率和ACC氧化酶活性,延缓果实硬度和可滴定酸含量下降。虽然不同成熟度的果实呼吸高峰和乙烯释放速率到达平台期的时间不同,但1-MCP处理对成熟度不同的果实品质影响无明显差异。(3)在0℃1 80d的贮藏过程中,对照果实出现了37.1、18.0、16.6kD三条特异蛋白条带,0.5μL·L-11-MCP处理显著抑制37.1、18.0、16.6kD新蛋白质的表达,推测这些蛋白质与苹果衰老有关并受乙烯调控。更多还原

【Abstract】 The storage behavior of‘Pink Lady’apples picked at 3 mature stages and the effects of 0.5μL·L-11-methylcyclopropene (1-MCP) on respiration, ethylene production, ACO (ACC oxidase) activity, storage quality and soluble proteins in apples with different maturity were studied during 6-months cold storage. The results were as follows.(1). Harvest maturity influenced storage quality of‘Pink Lady’apples stored at 0℃, especially when the fruits were stored over 3 months. The more mature the fruits were harvested, the more loss happened in flesh firmness, titratable acids and soluble solids after long-term storage. Therefore, it was recommended that the fruit expected for a long-term storage be harvested at the late middle of October and the fruit expected for a short-term storage be harvest by the end of October.(2). 1- MCP treatment (0.5μL·L-1 at 20℃for 24 hour) significantly inhibited the respiration, ethylene (C2H4) production and ACO activity, and slowed down the decrease in the flesh firmness and titratable acids of‘Pink Lady’apples during storage at 0℃. Some differences in the respiratory and ethylene peaks of fruits picked at three harvest dates were found. However there was no difference in the effects of 1-MCP on storage quality of 3-harvested fruits.(3). SDS-PAGE analysis of soluble proteins showed three new protein bands, 37.1, 18.0, 16.6kD, in untreated fruit during 6-months storage at 0℃. The appearance of these proteins were delayed in 1- MCP (0.5μL·L-1 )treated fruits. It is speculated that those new proteins are related to the apple senescence and their synthesis in apples is regulated by ethylene.更多还原