【作者】 林燕妮；

【导师】 吴国欣；

【作者基本信息】 福建师范大学 ， 生物化学与分子生物学， 2007， 硕士

【摘要】 本文介绍了良性前列腺增生症的发病机理及其治疗的方法，着重讨论了传统中药白芥子治疗前列腺增生的临床应用和药理研究的概况。首次报道了白芥子对前列腺增生模型小鼠前列腺细胞形态学以及细胞凋亡的影响，对开发治疗前列腺增生症的中药新药具有重要意义和应用价值。本论文从白芥子中分离出4种药效成分包括芥子碱、芥子苷、β—谷甾醇、挥发油，并进行含量测定。采用正交实验优化白芥子中芥子碱的提取，探讨微波、超声波辅助提取对提取效率的影响，并对芥子碱的含量测定方法进行比较研究。在药理研究方面，通过皮下注射丙酸睾酮建立良性前列腺增生症的动物模型，灌胃给药21天，分析各试验组动物的前列腺湿重指数和体积指数。制作前列腺石蜡切片并进行HE染色，光镜下观察各试验组小鼠前列腺组织的形态特征，探讨白芥子各药效成分对小鼠前列腺增生组织细胞的形态学影响。通过TUNEL、透射电镜以及DNA琼脂糖电泳多种方法来检测白芥子各药效成分对小鼠前列腺组织中细胞凋亡的影响，探讨其与细胞凋亡的相关性。结果表明芥子碱、芥子苷、β谷甾醇、挥发油对丙酸睾酮所致小鼠试验性前列腺增生症具有明显治疗和预防作用，并对增生的前列腺组织的病理形态具有改善作用。其治疗前列腺增生的机理之一是促进前列腺细胞凋亡，从而使前列腺体积缩小、重量减轻。更多还原

【Abstract】 This paper introduced the mechanism and therapy of benign prostatic hyperplasia(BPH), It discussed the clinic and pharmacological research of traditional Chinese medicinal herbs Semen Sinapis AIbae in treating BPH. This paper firstly elaborated the influence of Semen Sinapis Albae on the prostate cell morphology and the cell apoptosis of BPH in experimental mice. It supplied signify purport and applied cost for the exploitation of new traditional Chinese medicinal herbs in the treatment of BPH.This paper dealt with the extraction and content determination of four effective substances: sinapine, sinalbin,β-sitosterol and volatile, which extracted from Semen Sinapis Albae. The orthogonal test were applied to optimize the extraction of sinapine from Semen Sinapis Albae, and investigate the effect of microwave, ultrasonic—assisted extraction on the extraction efficiency of sinapine. The method of content determination of sinapine was also discussed in this paper.In pharmacological research, BPH animal model was built trough hypodermic inject testosterone propionate, intragastric administration for 21 days, prostatic weight index and prostatic volume index of every experimental mice were calculated. Prostatic paraffin section was made to performance HE Taining. To investigate the effect of four effective substances on the prostate cell morphology of BPH in experimental mice, prostate cell morphology was observed under light microscope. TUNEL、transmission electron microscopy and DNA agarose gel electrophoresis were applied to detect the influence of four effective substances on the apoptosis of the prostate cells in experimental mice. The correlation between the two was also explained in the paper. The results as follows: Four effective substances had a significant therapeutc effects on experimental mice with BPH. They can promote the apoptosis of the prostate cells and as a result, the volume and weight of the prostate of the mice were reduced.更多还原