【作者】 徐义荣；

【导师】 白莉；

【作者基本信息】 山西医科大学 ， 皮肤病与性病学， 2007， 硕士

【摘要】 目的:研究扁平苔藓（lichen planus LP）皮损中细胞凋亡的情况,探讨caspase-9、Apaf-1在扁平苔藓皮损中的表达和意义,分析二者的表达与扁平苔藓中细胞凋亡的关系。方法:应用原位末端标记技术（TUNEL法）及免疫组化的方法检测30例LP皮损和30例正常皮肤组织中的细胞凋亡的情况及caspase-9和Apaf-1蛋白的表达部位和表达强弱;采用RT-PCR的方法检测caspase-9mRNA在10例LP皮损和8例正常皮肤组织中的表达情况。结果:LP中表皮层角质形成细胞的凋亡指数（AI）75.67±9.4,明显高于正常对照组38.15±9.6（P＜0.01）;LP中Caspase-9和Apaf-1的阳性单位分别为14.45±2.72和11.93±3.14,均明显高于正常对照组（P＜0.01）;而且LP中caspase-9mRNA的表达量2.304±0.417与正常对照组1.605±0.381比较差别有统计学意义（P＜0.05）。LP中caspase-9、Apaf-1的阳性表达强度均与其自身的角质形成细胞的AJ呈正相关r₁=0.965 r₂=0.939（P＜0.01）。结论:LP中角质形成细胞凋亡亢进;线粒体凋亡途径的凋亡因子caspase-9、Apaf-1与角质形成细胞的凋亡关系密切;二者介导的线粒体凋亡途径可能参与了LP的发病过程。更多还原

【Abstract】 Objiect: To study the apoptosis of the keratinocytes in lichen planus and examine the expression and distribution of caspase-9、Apaf-1 in lichen planus. Analysis the relationship between the expression of them and the events of cell apoptosis in lichen planus.Methods: TUNEL and Immunohistochemical technique were employed to study apoptosis of keratinocytes and the expression of caspase-9、Apaf-1 in 30 linchen planus and 30 normal skin specimens.Caspase-9 mRNA were observed by RT-PCR in 10 linchen planus and 8 mormal skin.Results:The keratinocytes apoptosis index （AI） 75.67±9.4 in lichen planus were increased than its 38.15±9.6 in normal contral（P<0.01）.Compared with the normal control group,positive units（PU） of caspase-9 and Apaf-1 was 14.45±2.72 and 11.93±3.14 which were upregulated in keratinocyte in lichen planus （P<0.01）;Caspase-9mRNA 2.304±0.417 were also increased in LP than its in nomal skin（P<0.05）;Both caspase-9 and Apaf-1 were direct correlation with their own keratinocytes apoptosis index r₁=0.965 r₂=0.939 （P<0.01） .Conclusion: The apoptosis of keratinocytes were significantly accelerated in lichen planus; Caspase-9 and Apaf-1 might be participated in the apoptosis of kerationcytes in LP cases; Mitochondria apoptosis pathway may contribute to the formation and progression of lichen planus.更多还原