【作者】 张俊鸿；

【导师】 田琳； 孙建娅；

【作者基本信息】 山西医科大学 ， 劳动卫生与环境卫生学， 2007， 硕士

【摘要】 目的探讨焦炉工外周血淋巴细胞热应激蛋白90、60及27(HSP90、HSP60和HSP27)的表达水平及其与外周血细胞损伤之间的关系及意义。方法选取某钢铁集团公司某焦化厂焦炉作业工人288名作为暴露组,根据作业环境监测结果和工作地点分为高剂量组172人,低剂量组116人;同时选取该焦化厂2km外、无有毒作业接触史的某医院医护人员和管理人员38名作为对照组。调查其一般情况、工作史、个人史等情况;对作业环境进行监测;同时取受试者的肘静脉血,应用Western blot法测定外周血淋巴细胞的HSP90、HSP60和HSP27水平;用ELISA试剂盒测定外周血清乳酸脱氢酶(LDH)活力和丙二醛(MDA)含量;用单细胞凝胶电泳法(SCGE,即彗星试验)测定外周血淋巴细胞DNA的损伤程度。结果各组调查对象在年龄、性别、吸烟、饮酒方面差异均无显著性(P＞0.05)。热应激蛋白表达结果显示,焦炉工高剂量组淋巴细胞HSP90水平(比值)(0.24±0.32)高于低剂量组(0.12±0.30)和对照组(0.06±0.33),具有统计学差异(P＜0.05);HSP60、HSP27各组之间未见统计学差异。LDH测定结果显示,焦炉工高剂量组血清LDH水平(246.7±42.79)U/L高于低剂量组(205.7+33.72)U/L和对照组(199.7±31.78)U/L,具有统计学差异(P＜0.05)。MDA测定结果也显示,焦炉工高剂量组血清MDA水平(2.68±0.67)nmol/ml高于低剂量组(2.35±0.58)nmol/ml和对照组(2.24±0.66)nmol/ml,具有统计学差异(P＜0.05)。彗星试验的各项结果显示,焦炉工人外周血淋巴细胞DNA损伤显著高于对照组,且高剂量组工人(炉顶、炉侧)的DNA损伤显著高于低剂量组的工人(炉底及辅助工)(P＜0.05)。经线性趋势x~2分析(x~2值为36.411,P＜0.001)和Spearman相关分析(r=-0.345,P＜0.001),随多环芳烃(PAHs)暴露水平的增高,DNA损伤程度也呈逐渐加重(即损伤级别增高)的趋势。经多元回归分析,彗星实验的Olive尾矩与焦炉工人的PAHs接触剂量存在着剂量—反应关系。(β=-0.117,P=0.030)结论1、淋巴细胞彗星试验结果显示,焦炉逸散物可导致遗传物质损伤。2、焦炉工外周血淋巴细胞HSP90等HSPs水平的增高可能起到保护细胞DNA免受致癌物PAHs损伤作用。3、焦炉工人血清中的LDH和MDA及外周血淋巴细胞中的HSP90可为PAHs暴露的生物标志物的研究提供科学依据。更多还原

【Abstract】 Objective To explore the relationship between the expression of heat shock protein 90,60 and 27(HSP90,HSP60 and HSP27)and cell damage in peripheral blood of workers exposed to coke oven emissions.Methods 288 coke oven workers in X steel factory were divided into high dose group and low dose group on the basis of environment monitoring result and work place.There were 172 men in high dose group and 116 men in low dose group.While 38 workers unexposed occupationally carcinogenic substances were selected as the control group,who were employed in medical therapy unit nearby 2 kilometers from steel factory.We investigated their general information,history of personal and occupational exposure,and the work environment.Blood samples were collected immediately after a shift at the end of a working day from 288 coke oven workers and 38 control workers.Levels of HSP90,HSP60 and HSP27 in peripheral blood lymphocytes were measured by Western blot.LDH and MDA were measured by ELISA. Chromosomal aberrations and the degree of DNA damage in peripheral blood lymphocytes were detected by comet assay.Results There were no significant differences among those groups regarding age,sex, smoking and drinking habits(P＞0.05).Levels of HSP90(ratio)in peripheral blood lymphocytes in three groups were separately 0.24±0.32,0.12±0.30 and 0.06±0.33.They increased significantly compared with that of the control.But levels of HSP60 and HSP27 were not significantly different among those groups.Monitoring result of LDH appeared that the level of LDH in the high dose group((246.7±42.79)U/L)was higher than those in the low dose group ((205.7±33.72)U/L)and in the control group((199.7±31.78)U/L)(P＜0.05).Detectable result of MDA presented that the level of MDA in the high dose group((2.68±0.67)nmol/ml)was bigger than those in the low dose group((2.35±0.58)nmol/ml)and in the control group ((2.24±0.66)nmol/ml)(P＜0.05).Compared with the control group,there was significant difference in tail length,Olive Tail Moment of occupational exposure workers,high dose group＞low dose group＞control group(P＜0.05).The degree of DNA damage increased with the rising of exposure BaP dose(χ~2=36.411,P＜0.001;Spearman r=-0.345,P＜0.001).There is a dose-effect relationship between Olive and exposed dose of PAHs.(β=-0.117,P=0.030)Conclusion 1.Result of comet assay showed that Coke oven emission could result in genetic damage.2.The increase of HSP90 level may be involved in protection of DNA from genotoxic damage induced by carcinogen.3.LDH and MDA in peripheral blood and HSP90 in peripheral blood of coke oven workers could offer scientific evidence for the study of an exposure biomarker to evaluate PAHs contact level.更多还原