【作者】 任婷婷；

【导师】 卢放根；

【作者基本信息】 中南大学 ， 消化内科， 2007， 硕士

【摘要】 目的：观察饮食性高脂血症大鼠大、小肠菌群变化，比较抗生素干预组与益生菌干预组血脂升高情况，探讨肠道菌群潜在的调脂作用。方法：50只SD大鼠随机均分为5组，A组喂养普通饲料，B、C、D、E组喂养高脂饲料，B组(抗生素干预组)于实验第1天起予甲硝唑、诺氟沙星灌胃建立肠道菌群缺陷模型，C组为阳性对照组，D(菌株1干预组)、E(菌株2干预组)于第15天起分别予菌株1、2灌胃，第30天观察结果。第1天、第15天、第30天取大鼠粪便行菌群分析；第1天、第30天测量血脂指标。第30天处死大鼠，取小肠内容物行菌群分析。结果：高脂饲料组(B、C、E)血胆固醇、LDL较其初始值明显增高，ΔTC明显高于A组(P＜0.05)。但菌株1干预组(D组)TC、TG增高较初始值无统计学意义，且ΔTC较A组无统计学差异。B、C、E组体重较A、D组明显增高(P＜0.05)，D组较A组无显著增高(P＞0.05)。高脂饮食组大便菌群发生明显改变，嗜酸乳杆菌、双歧杆菌、肠球菌计数明显降低(P＜0.05)，拟杆菌呈现“先增后降”趋势(P＜0.05)。小肠菌群相对稳定，C组拟杆菌较A组明显降低(P＜0.05)，D组小肠拟杆菌、肠球菌、大肠杆菌、金葡菌较A组明显降低(P＜0.05)。结论：1.1％胆固醇、5％鲜猪油、10％蛋黄粉、0.2％胆酸钠、84％基础饲料混合制成的高脂饲料连续喂养SD大鼠30天可建立高胆固醇、LDL—胆固醇血症模型。2．高脂饮食(30天)可导致肠道菌群结构的改变，以大肠菌群为主，这种改变可能反过来进一步促进高脂血症的发展。3．菌株1可能具有降血脂、抑制脂肪蓄积的潜在益生菌功效，作用点可能位于小肠。更多还原

【Abstract】 Objective:To observe the changes of both large intestinal and smallintestinal flora of hyperlipidemia SD rats, compare the elevation of serumlipid of antibiotics intervention group and bacteria strain interventiongroup in order to approach the latent capability in accommodating serumlipid of intestinal flora.Method:50 SD rats were randomly divided into 5 groups. Group Awere normally fed,while Group B、C、D、E were fed by forage rich in oiland fat. Group B received antibiotics intervention by arilin andnorfloxacin intragastric administration from the 1st day on. Group D andE had been receiving intragastric administration of bacteriaⅠandⅡrespectively since the 15th day. We analyse the fecal microbial populationat the 1st day, the 15th day, and the 30th day, survey the serum lipid at the 1stand the 30th day. On the 30th day, we executed the rats and analyse thesmall intestinal flora.Results:The level of TC、LDL was elevated significantly inGroupB、C、E compared to its primary concentration,the increment ofTC(ΔTC) in these groups was higher than GroupA (P＜0.05) .InGroupD,the elevation of TC、TG was not significant as well asΔTC(P＞0.05).The increase of bodyweight in GroupB、C、E was significantlyhigher than GroupA, while GroupD was not apparently higher thanGroupA (P＞0.05) .The fecal microbial population changed a lot in GroupB、C、D、E,the counts of Lactobacillus acidophilus、Bifidobacteria、Enterococci was significantly decreased,the counts of Bacteroides wasalso decreased after a increasing period (P＜0.05) .The small intestinalflora was relatively stable,with just Bacteroides decreased in GroupC. Butin groupD,the counts of Bacteroides、Enterococci、Escherichia coli andStaphylococcus aureus was significantly decreased compared to GroupA.Conclusion:1.Feeding the SD rats with mixed forage made of 1% cholesterol、5% animal oil、10%egg yolk powder、0.2% Sodium Cholate and 84% basicforages for 30 days can establish hyperlipidemia rats model.2.High lipid diet intake for 30 days can result in the changes ofintestinal flora which mainly occurred in large intestine and wasconsidered to possibly accelerate the development of hyperlipidemia.3.Bacteria strain I may have the latent ability to affect lipid and fatmetabolism in small intestine,and was considered to be a candidateprobiotic.更多还原