【作者】 宋冬灵；

【导师】 毛培宏； 曾宪贤；

【作者基本信息】 新疆大学 ， 核技术及应用， 2007， 硕士

【摘要】 离子注入白金针菇及其生物学效应的研究在国内还是首次,具有一定的创新性。本论文的研究目的是研究离子注入对白金针菇的生物学效应的影响,为获得优质、高产的金针菇菌株提供较佳的参数和新的出发菌株,为食用菌育种的学术研究提供重要的参考价值。在本文中,通过正交优化试验确定出了白金针菇菌丝生长的最佳固体培养基配方,液体发酵试验筛选出了较好的液体发酵配方。在此基础上对白金针菇F3进行了三次N+离子注入。注入结果表明,N+离子注入对白金针菇的生物学效应明显,不同注入剂量的生物学效应不同,呈“马鞍型”曲线变化规律。同时,离子注入会导致白金针菇菌丝细胞发生突变,且突变率高,突变谱宽。通过一系列筛选工作和生物学效应的分析对比,获得了与出发株相比有明显变异特征的正突变株F6、F615和负突变株F62。对他们进行了出菇试验,结果突变菌株F6、F615第一茬菇的平均生物转化率比出发株提高了24.01%,出菇周期缩短了15d,均盖直径小了0.3cm。而突变菌株F62的出菇情况则相反,第一茬菇的平均生物转化率仅为35.38%,比出发菌株下降了39.75%,出菇周期长达97d。最后,总结了一些出菇栽培过程中的经验,为工厂化生产提供一些参考。更多还原

【Abstract】 The research on biological effects of white flammulina velutipes implantated by nitrogen ion beam was studied for the first time at home and there was some innovations. The intention of this paper was to study the biological effects of white flammulina velutipes implantated by nitrogen ion beam and to offer some important parameters and new strains for the flammulina velutipes with good propoties and high yields. There was also some excellent reference values for the scientifical study of edible mushroom breeding.In the paper, the best prescription constituents of solid medium to the mycelium growth of white flammulina velutipes was founded by orthogonality optimization experiment and the better prescription constituents of liquid fermentation medium was screened out by liquid fermentation experiment. On this base, the white flammulina velutipes F3 was implanted by nitrogen ion beam three times. The results indicated that there was remarkable biological effects on the white flammulina velutipes implanted by nitrogen ion beam and the effects were different with different doses. The relation between the doses and the survival rate was“saddle modle”curve. At the same time, the mycelial cells was induced by nitrogen ion beam implantation. And the mutation rate was high, the mutation spectrum was wide. The positive mutation strain F6, F615 and the negative mutation strain F62 which had remarkable characters compared with the control strain (F3) were finally screened out through a series of filter work and contrast analysis of biological effects.The average biotransformation rate for the first fruit of F6, F615 was 24.01% higher than that of the control strain, the fruit cultivation periods was shorter and the diameter of pileus was 0.3 centimeters smaller than that of the control strain. The the mutation strain F62 was the reverse. The average biotransformation rate of F62 for the first fruit was only 35.38% which was 39.75% lower than that of the control strain. The fruit cultivation periods of F62 lasted for 97days. Finally, some experience of the growth and cultivation were sumed up and some referrences for industrialization production were supplied.更多还原