【作者】 白如鑫；

【导师】 李沛；

【作者基本信息】 福建中医学院 ， 针灸推拿学， 2007， 硕士

【摘要】 目的：对中老年男子部分雄激素缺乏综合征(PADAM)SD大鼠模型进行可行性探索，观察温和灸法对(PADAM)模型大鼠的疗效及生殖内分泌的影响。方法：参照文献方法，根据测定的大鼠血清总睾酮(TT)和血清游离睾酮(FT)的水平，选择符合标准的PADAM的中老年雄性SD大鼠模型30例按照随机分为3组，观察组(灸法组)、对照组Ⅰ(丙酸睾酮组)、对照组Ⅱ(空白对照组)，并通过比较各组疗程前后血清总睾酮和游离睾酮的水平、观察各组大鼠一般情况的变化、悬尾实验和力竭游泳实验及取得的相关脏器指数评价大鼠对干预手段的反映情况。结果：(1)疗程前三组大鼠血清中的总睾酮(T)和游离睾酮(FT)水平无显著性差异(P＞0.05)，具有可比性。(2)疗程后观察组和对照组Ⅰ血清中总睾酮的含量较前均有显著性提高，其中对照组Ⅰ提高较为显著(P＜0.01)，观察组(P＜0.05)，两组之间无显著性差异(P＞0.05)，而对照组Ⅱ无显著性变化，但有下降趋势(P＞0.05)。(3)疗程后观察组和对照组Ⅰ血清中游离睾酮的含量较前均有显著性提高(P＜0.05)，两组之间无显著性差异(P＞0.05)，而对照组Ⅱ血清中游离睾酮的含量显著下降(P＜0.05)。(4)疗程前悬尾实验三组大鼠不动时间无显著性差异(P＞0.05)，具有可比性。观察组与对照组Ⅰ疗程前后比较不动时间有非常显著性缩短(P＜0.05)，对照组Ⅱ组疗程前后比较无显著性变化(P＞0.05)。疗程后观察组和对照组Ⅰ之间无显著性差异(P＞0.05)。(5)大鼠力竭游泳实验时间比较，观察组与对照组Ⅰ无显著性差异(P＞0.05)，观察组、对照组Ⅰ与对照组Ⅱ比较时间有显著性延长(P＜0.01)。(6)肾周脂肪、脾脏、胸腺、睾丸脏器指数观察组与对照组Ⅰ均无显著性差异(P＞0.05)，观察组在肾周脂肪、睾丸脏器指数上显著高于对照组Ⅱ(P＜0.05)，胸腺脏器指数最为明显(P＜0.01)，而脾脏脏器指数无显著性差异(P＞0.05)；对照组Ⅰ胸腺、睾丸脏器指数上显著性高于对照组Ⅱ(P＜0.05)，肾周脂肪脏器指数增高最为明显(P＜0.01)，脾脏脏器指数亦无明显差异(P＞0.05)。(7)脾脏脏器指数与血清TT、FT无显著相关性(p＞0.5)；胸腺脏器指数与血清TT、FT呈显著正相关(P＜0.01)；睾丸脏器指数与血清TT、FT呈显著正相关(p＜0.01)；肾周脂肪脏器指数与血清TT、FT呈显著负相关(p＜0.01)；大鼠悬尾实验时间与血清TT呈显著负相关(p＜0.01)；大鼠力竭游泳时间与血清TT、FT呈显著正相关(p＜0.01)。结论：(1)通过测定大鼠血清的总睾酮(TT)和血清游离睾酮(FT)的水平可以建立出符合中老年男子部分雄激素缺乏综合征(PADAM)的大鼠模型。(2)目前还没有证据表明单一检测一种激素水平可以替代检测总睾酮和游离睾酮水平来建立模型。(3)温和灸对于PADAM大鼠模型的作用效果与丙酸睾丸素作用效果平行，可以改善模型大鼠的体能、抑郁状态、脂肪堆积等更年期相关症状，且与下丘脑-垂体-睾丸轴的调节有关。提示：单纯温和灸可以作为睾酮补充疗法的替代疗法之一进行PADAM的临床研究。更多还原

【Abstract】 Purpose: Research the feasibility by establishment of simutaneous PADAMmodel in SD rats. To observe the effect and the influence of reproductiveendocrine for the rats models of PADAM by the therapeutic of Mild Moxibustion.Method: Based on the methods after being established, sellected 30 samplesof simutaneous PADAM model in rats according to the serum level of the totaltestosterone (TT) and free testosterone(FT),and divided into 3 groups accordingto random principle, the observation group(mild moxibustion group)、controlgroupⅠ(the testosterone propionate group) and the control groupⅡ(blankgroup). By comparing the level of the serum total testosterone and freetestosterone before and after therapeutic in each group, observing the variationof the general status of the rats, and exhaustive swimming exercise,tailsuspension test and the correlative visceral organs indexes, estimated thereflect condition of intervention instrument in model rats.Results:(1)before therapeutic, the serum level of the total testosterone(TT)and free testosterone(FT) among the three groups was the samesignificance(P＞0.05), there was a comparison. (2)After therapeutic, the serumlevel of the total testosterone and free testosterone of the observation groupand control groupⅠwere both significant higher than the pretherapy, controlgroupⅠimproved more(P＜0.01), observation group(P＜0.05),statistics show thesame significance between them(P＞0.05). The control groupⅡdid not droppedremarkably for the serum level of the total testosterone(P＞0.05). (3) Aftertreatment, the serum level of the free testosterone(FT) of the observation groupand control groupⅠimproved remarkably than the pretherapy(P＜0.05),but statistics show the same significance between them(P＞0.05), control groupⅡdropped remarkably(P＞0.05). (4) For the immobility time of tail suspension test,before treatment, among the three groups was the same significance(P＞0.05),there was a comparison. After treatment, observation group and control groupⅠare both significant shorter than the pretherapy(P＜0.05), the control groupⅡdid not changed remarkably(P＞0.05).Statistics show the same significancebetween observation group and control groupⅠ(P＞0.05) after treatment. (5)Andfor the time of the exhaustive swimming exercise, observation group and controlgroupⅠare was the same significance(P＞0.05),and both significant longer thanthe control groupⅡ(P＜0.01). (6) For the correlative visceral organs indexes,observation group and control groupⅠwere similar(P＞0.05).For perirenal fatand testis, observation group was significant higher than the control groupⅡ(P＜0.05),thymus was even more marked(P＜0.01),but for spleen,they weresimilar(P＞0.05). For the thymus and testis, control groupⅠwas significanthigher than the control groupⅡ(P＜0.05), the perirenal fat was even moremarked(P＜0.01),but for spleen,they were also similar (P＞0.05). (7) Visceralorgans index of spleen was no correlated with TT、FT(P＞0.05);there wassignificant positive correlation between visceral organs index of thymus andTT, FT(P＜0.01);so to visceral organs index of testis and TT、FT(P＜0.01); therewas significant negative correlation between visceral organs index of theperirenal fat and TT, FT(P＜0.01); so to the time of tail suspension test andTT, FT(P＜0.01);there was significant positive correlation between exhaustiveswimming exercise and TT, FT(P＜0.01).Conclusion:(1) Simutaneous PADAM model in rats can be established accordingto the menstruation the serum level of the total testosterone and freetestosterone. (2) At the present time, there is no attestation shows thatsimutaneous PADAM model in rats can be established according to singlemenstruation the serum level of the total testosterone or free testosteroneinstead both. (3)The effect of the moxibustion and testosterone propionate onthe models of PADAM is similar. It can improve the rats’ correlation climactericsymptom of physical ability, depressive state, abdominal adipose accumulation etc, and were relative to the functional of the hypothslsmic—pituitary—testicle axis. So it implies that mild moxibustion can be used as one of thereplacement therapies to testosterone complementary therapies for clinicalresearch of PADAM.更多还原