【作者】 李莉；

【导师】 王守彪；

【作者基本信息】 青岛大学 ， 人体解剖与组织胚胎学， 2006， 硕士

【摘要】 目的研究磷酸化信号传导和转录激活因子3和端粒酶逆转录酶在脂溢性角化病中的表达及其意义。方法选择鳞状细胞癌、脂溢性角化病、正常皮肤各20例，运用免疫组织化学方法，观察细胞细胞中磷酸化信号传导和转录激活因子(PSTAT3)和端粒酶逆转录酶(hTERT)的表达水平，并进行比较。结果1、磷酸化信号传导和转录激活因子3(PSTAT3)在20例脂溢性角化病皮损表达中，14例表达呈阴性，6例表达阳性，阳性率30％；20例鳞状细胞癌皮损中，12例表达呈阴性，8例表达呈阳性，阳性率40％；20例正常皮肤组织中均为阴性表达。磷酸化信号传导和转录激活因子3(PSTAT3)在恶性皮肤肿瘤中的阳性表达率明显高于脂溢性角化病(P＜0.05)；而在脂溢性角化病中的阳性表达率明显高于正常皮肤(P＜0.05)。2、端粒酶逆转录酶(hTERT)在20例脂溢性角化病皮损表达中，14例表达呈阴性，6例表达阳性，阳性率30％；20例鳞状细胞癌皮损中，10例表达呈阴性，10例表达呈阳性，阳性率50％；20例正常皮肤组织中，19例表达呈阴性，1例表达呈阳性，阳性率为5％。端粒酶逆转录酶(hTERT)在恶性皮肤肿瘤中的阳性表达率明显高于脂溢性角化病(P＜0.05)；而在脂溢性角化病中的阳性表达率明显高于正常皮肤(P＜0.05)。结论1、磷酸化信号传导和转录激活因子PSTAT3和端粒酶逆转录酶hTERT与肿瘤的发生发展及增殖程度密切相关；2、脂溢性角化病是一种有潜在恶变倾向的良性肿瘤。更多还原

【Abstract】 Objective To investigate the expression and significance of Signal transducer andactivator of transcription 3(PSTAT3) phosphorylation and Human TelomeraserReverse Transcriptase (hTERT) in Seborrheic Karatosis.Methods Immunohistochemistry technique was employed to measure theexpression of PSTAT3 and hTERT in skin tissue from 20 cases of skin squamous cellcarcinoma(SCC), 20 cases of seborrhoeic keratosis(SK) and 20 normal subjects.Results 1. 6 cases of SK and 8 cases of SCC expressed PSTAT3 protein and therewas no PSTAT3 protain expression on normal skin. Expression of PSTAT3 in SCCwas at a higher level than that of SK, also, the difference of expression of PSTAT3 hasobvious significance between SK and normal skin.2. 6 cases of SK, 10 cases of SCCand 1 case of normal skin expressed hTERT. Expression of hTERT in SCC was at ahigher level than that of SK, also, the difference of expression of PSTAT3 has obvioussignificance between SK and normal skin.Conclusion 1. The levels of expression of PSTAT3 and hTERT was associatedclosely with the criticial stages in cutaneous tumor in development.2. seborrhoeickeratosis(SK) is a kind of benign skin tumor, but it has potential cutaneouscarcinomas formation.更多还原