【作者】 陈玉明；

【导师】 夏玉军；

【作者基本信息】 青岛大学 ， 人体解剖与组织胚胎学， 2007， 硕士

【摘要】 目的探讨兔大脑中动脉缺血再灌注(MCAO／R)后脑组织神经元特异性烯醇化酶(NSE)和S-100蛋白的表达及其血清含量的变化。方法将制作成功的兔MCAO／R模型58只，随机分为永久性缺血组30只和缺血再灌注组28只；另取10只动物行假手术分别作为缺血组(5只)及再灌注组(5只)的对照组。免疫组化法检测脑缺血再灌注不同时间脑组织NSE和S-100蛋白的表达，ELISA法检测血清NSE和S-100的含量。结果假手术组脑组织NSE和S-100表达微弱，血清含量较低。永久性脑缺血3h和缺血再灌注2h后，脑组织NSE和S-100表达同步升高和降低、变化趋势基本一致，但NSE和S-100表达在缺血再灌注47h组较永久性缺血48h组再次明显升高。血清NSE和S-100水平直到缺血5h和再灌注5h开始同步升高，较脑组织NES和S-100表达时间延迟3h。结论神经细胞和胶质细胞对缺血再灌注损伤非常敏感，再灌注可加重脑组织的损伤，破坏血脑屏障，NSE和S-100从脑脑脊液循环进入了血液循环，血清NSE和S-100的水平可作为评价脑损伤程度和转归的早期指标。更多还原

【Abstract】 Objective To observe the expressions and serum levels of neuron-specific enolase(NSE) and S-100 after middle cerebral artery occlusion and reperfusion (MCAO/R) inrabbits.Methods fifty-eight successful MCAO/R rabbit models were randomly divided intopermanent ischemic group 30 cases and ischemic reperfusion group 28 cases. Another10 rabbits were regarded as ischemic congtrol (5 cases) and reperfusion control (5cases). The expressions of NSE and S-100 in the brain tissue were determined withimmunochemical assay, and the serum levels of NSE and S-100 were measured withezyme linked immunosorbent assay (ELISA).Results There were weak expressions and low serum levels of NSE and S-100 issham group. The expressions of NSE and S-100 increased/decreased and changedbasically as a same principles following permanent ischemic 3h and ischemicreperfusion 2h. But the expressions of NSE and S-100 in reperfusion 47h were alsohigh than that in permanent ischemic 48h. The serum levels of NSE and S-100increased same at ischemic 5h and reperfusion 5h, which were delayed 3h than that inbrain tissue expression.Conclusion Neurons and glia were much sensitive to ischemic reperfusion. Thereperfusion might enhance brain tissue injury and destroy blood brain barrier to causethe NSE and S-100 into blood from cerebral spinal fluid, so that the serum levels ofNSE and S-100 might serve as early indexes evaluating brain injury and outcome.更多还原