【作者】 蔡丙严；

【导师】 丁铲；

【作者基本信息】 扬州大学 ， 预防兽医学， 2007， 硕士

【摘要】 新城疫病毒(Newcastle disease virus,NDV)严重危害了各国养禽业的发展。目前国内外均采用疫苗免疫接种进行预防,尚无有效的治疗方法,而中药在新城疫的防治上越来越受到人们的重视,并取得了显著的科技成果。本研究选取临床上常用的抗病毒中药成分氧化苦参碱(oxymatrine, OM)、甘草酸(glycyrrhizic acid, GA)、绿原酸(chlorogenic acid, CA)、栀子苷(geniposide, GP)、虎杖苷(polydatin, PD)、木犀草素(luteolin, LT)、芦丁(rutin, RT)、黄芪多糖(astragalus polysaccharide, APS)作为研究对象,采用细胞培养法体外分两次筛选抗病毒活性的中药单体和复方成分,通过动物实验验证体外的筛选结果。体外初筛出了四种单体和四种复方,其抗毒活性依次为OM>APS>GA>GP;方1(OM+APS)>方3(OM+APS+GP)>方2(APS+GA)>方4(APS+GA+GP);复筛出两种单体和两种复方,其抗毒活性依次为OM>APS;方1(OM+APS)>方3( OM+APS+GP )。动物实验表明单体OM对NDV有显著的预防作用,而复方1则有显著的治疗作用,与攻毒对照比较,实验组表现为雏鸡死亡率低、器官病变轻微、增重较显著。为寻找优质的抗NDV活性物质和研制新的防治ND中药制剂提供了理论依据,对ND的临床防治具有重要意义。更多还原

【Abstract】 Newcastle disease virus (NDV) seriously damaged to the poultry industry developing all over the world. At present, the most effective control method is vaccination for prevention.In this test, a few of antiviral Chinese traditional medicines were selected, such as Oxymatrine (Oxymatrine, OM),Glycyrrhizin (glycyrrhizic acid, GA),Chlorogenic acid (chlorogenic acid, CA), Geniposide(geniposide, GP), Polydatin (polydatin, PD), Luteolin (luteolin, LT),Rutin (RT) and Astragalu polysaccharide (Astragalus polysaccharide, APS). Screening of monomer and compound which had inhibitivity active to NDV in vitro and in vivo.The antiviral activity of four monomers and recipes showed as follow:OM>APS>GA >GP;recipe1(OM+APS)>recipe3(OM+APS+GP)>recipe2(GA+AP )>recipe4(GA+APS+GP) at former screening, and OM>APS;recipe1(OM+APS)>recipe3 (OM+APS+GP) at aga- in screening in vitro.The animal results showed that OM had significant prevention for ND,however, recipe1 had significants treatment for ND. The group of exprements showed that a higher protection ratio, less organ lesions and more remarkable enhancing in weight compared with group of virals control. It would be able to provide a therorational basis for new Chinese medicine reagents, as well as those drug for clinical prevention and treatment of NDV innovation.更多还原