莱菔子水溶性生物碱对自发性高血压大鼠降压和早期肾保护作用的实验研究

【作者】 张旭生；

【导师】 盖国忠；

【作者基本信息】 长春中医药大学 ， 中医内科学， 2007， 硕士

【摘要】 目的：本文在中医理论指导下，结合现代研究，观察探讨莱菔子水溶性生物碱降压作用及保护自发性高血压大鼠(SHR)早期肾损害的机理。方法：将40只SHR随机分为5组：空白对照组、莱菔子水溶性生物碱高、中、低剂量组、卡托普利组，每组8只。采用尾动脉测压法每两周测一次血压，于给药8周后，摘取肾脏，制作肾组织石蜡切片，HE染色，光镜下观察大鼠肾小动脉的变化，并测量每张切片肾小动脉壁厚／腔径的比值，取均值。结果：给药8周后，莱菔子水溶性生物碱明显降低了SHR的血压，其中高、中剂量组的降压幅度与卡托普利组比较无显著性差异，同时莱菔子各组和卡托普利组均使SHR的肾动脉得到了一定程度的改善。结论：高血压早期肾损害主要发病机制为肝阳上亢，痰浊阻肾。因此立“降气化痰，通腑泻浊”作为主要治法。研究证实莱菔子水溶性生物碱具有明显的降压作用，同时对高血压引起的早期肾损害具有保护作用。更多还原

【Abstract】 Objective:under the direction of traditional Chinese medicine(TCM),combining modern research,to observe and discuss the mechanism of raphani soluble alkaloid on depressurization and protection for early-stage kidney damage of spontaneous hypertensive rat(SHR).Method : 40 SHR were randomly divided into 5 groups: control group, raphani soluble alkaloid groups with high,middle or low dosage respectively, captopril group. correspondingly 8 SHR for each group. blood pressure was tested every two weeks by caudal artery manometry, 8weeks after administration, animals were fasted for 12 hours,then their abdomens were opened, furthermore they were put to death by haemospasia and their kidney artery were cut and put into fixative solution containing 10% formaldehyde .24 hours after fixation, wax section and HE dying were prepared for observing the change of kidney arteriole under light microscope, at the same time , the ratio of thickness to cavity diameter for each section was measured and mean value was calculated. Result: 8weeks after administration, raphani soluble alkaloid could lower the blood pressure of SHE obviously, among which high and middle dosage groups manifested no significant difference from captopril group, while each group of raphani soluble alkaloid showed improvement on kidney arteriole of SHE to some extent .Conclusion : the main mechanism of early-stage kidney damage along with hypertension is hyperactivity of liver-YANG, muddy sputum hindering kidney. So "lowering adverse-rising energy and dissipating phlegm, FU-organ smoothness and turbidity evacuation" should be applied as major treatment method .research has proved that raphani soluble alkaloid has conspicuous depressurization and give protection role to early-stage kidney damage aroused by hypertension.更多还原