【作者】 王文强；

【导师】 张继；

【作者基本信息】 西北师范大学 ， 植物学， 2007， 硕士

【摘要】 本论文包括四部分:第一章固定化细胞技术及其应用研究进展本章综述了固定化细胞的方法、载体材料及其应用,详细阐述了常用固定化细胞的载体材料,固定化细胞载体材料的改进,固定化细胞在食品与发酵工业、化学与医药工业、能源开发和环境工程上的应用,总结了固定化细胞的优异性能,并对未来的发展给予展望。第二章酿酒酵母的分离鉴定本章利用富集培养基、分离培养基、鉴定培养基等,通过稀释涂板法、平板划线分离法,从土壤中分离出1株酿酒酵母菌。对它的生理生化性质进行研究,并做了初步的鉴定,鉴定为内孢霉目,酵母属,酿酒酵母。第三章固定化细胞材料的制备及其性能研究本章包括两节:第一节卡拉胶、瓜儿豆胶和膨润土固定化细胞材料的制备和性能研究首次以卡拉胶、瓜儿豆胶和膨润土为载体固定化酿酒酵母,制备出具有高强度的卡拉胶/瓜尔豆胶/膨润土复合材料,利用正交设计优化了复合材料制备条件,并利用机械强度、扫描电镜、X射线衍射、红外、热分析对此复合材料进行了表征。第二节卡拉胶、瓜儿豆胶和有机膨润土固定化细胞材料制备及其性能的研究首次利用卡拉胶、瓜儿豆胶和有机膨润土为载体制备固定化酿酒酵母,制备了一种新型的卡拉胶/瓜尔豆胶/有机膨润土复合材料,利用正交设计优化了复合材料制备条件。并利用机械强度、扫描电镜、X射线衍射、红外、热分析对此复合材料进行了表征。第四章固定化酿酒酵母细胞的制备及性能研究制备了固定化酿酒酵母进行了酒精发酵试验,确定了其最佳发酵发酵条件。通过电镜可以看到复合材料的表面和内部均有大量的酿酒酵母细胞生长,用机械强度,SEM、IR等对固定化细胞进行了表征。通过试验室发酵试验得出酿酒酵母菌的最佳发酵条件:温度为30℃,pH值为5,时间为72h时固定化细胞发酵酒精的最大产率为15.1%更多还原

【Abstract】 This article includes four parts:Part I: A review on research and development of technology of immobilized cells and their applications.In this part, the method of immobilized-cell preparation, the support of immobilized cells and their applications were looked back. Frequently used-support of immobilized cells and the application of immobilized cells in foodstuff and fermentation industry, chemical and medical industry, energy sources and environment engineering were discussed. Frequently used-support and the improvement on support of immobilized cells were emphasized. The excellent properties of immobilized cells and bright future were given.Part II: Isolation and identification of Saccharomyces cerevisiaeIn this part, Saccharomyces cerevisiae, which can produce alcohol, were isolated from the foot soil of maize. And also it was identified.Part III: Studies on preparation and characterization of immobilized materialThis part includes two subsection:Section one: Studied on Preparation and characterization of montmorillonnite/ carrageen/ guar gum gel spherical beadsA new way to prepare composite gel spherical beads using montmorillonnite as a modifier was carried out. The beads were characterised by determining their mechanical strength, and by X-ray diffraction (XRD) and FT-IR spectroscopy. Their thermal stability and swelling kinetics were also determined.Section two: Studied on Studied on Preparation and characterization of quaternary-montmorillonnite/ carrageen/ guar gum gel spherical beads.A new way to prepare composite gel spherical beads using quaternary-MMT as a modifier was carried out. The beads were characterized with SEM, machine intensity, XRD and FTIR. The thermal stability of the composite gel spherical beads was also identified.Part IV : Studies on preparation and characterization of living Saccharomyces cerevisiae cell immobilizationThis study carried on the first step research to the possibility that turns living Saccharomyces cerevisiae cell immobilization. And the effect of storage time on the cell activity was also studied. And the optimum conditions of fermentation were determined. The temperature is 30℃, pH is 5, time is 72h.更多还原