【作者】 梁延省；

【导师】 陈国强；

【作者基本信息】 汕头大学 ， 生物化学与分子生物学， 2007， 硕士

【摘要】 三聚物P(3-hydroxybutyrate-co-3-hydroxyvalerate-co-3-hydroxyhexanoate)(PHBVHHx)是通过重组菌Aeromonas hydrophila发酵获得的，其包括5.3 mol％3-hydroxyvalerate(3-HV)和10.2 mol％3-hydroxyhexanoate(3-HHx)。本文第一次在体外通过与poly(L-lactic acid)(PLA)，poly(3-hydroxybutyrate)(PHB)，poly(3-hydroxybutyrate-co-3-hydroxyvalerate)(PHBV)and poly(3-hydroxybutyrate-co-3-hydroxyhexanoate)(PHBHHx)的对比研究三聚物PHBVHHx的生物相容性。扫描电镜结果表明只有在PHBVHHx上才具有螺纹状的形态结构。噻唑蓝检测证明三聚物PHBVHHx在促进小鼠成纤维细胞L929和成骨细胞MC3T3的粘附和繁殖方面都分别比其他四种多聚物材料强。兔子体内组织学研究表明PHBVHHx是一个温和无伤害的生物植入材料。因此，PHBVHHx其可塑的物化性质和其生物相容性都使它可能成为新一代的生物植入材料。更多还原

【Abstract】 Terpolyester poly(3-hydroxybutyrate-co-3-hydroxyvalerate-co-3-hydroxyhexanoat)(PHBVHHx) containing 5.3 tool% 3-hydroxyvalerate (3-HV) and 10.2 tool%3-hydroxyhexanoate (3-HHx) was obtained via microbial synthesis using recombinantAeromonas hydrophila. For the first time in vitro biocompatibility of the terpolyesterwas evaluated in comparison with poly(L-lactic acid) (PLA), poly (3-hydroxybutyrate)(PHB), poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) andpoly(3-hydroxybutyrate-co-3-hydroxyhexanoate) (PHBHHx). Scanning electronmicroscopy (SEM) showed whorls-like morphology only on PHBVHHx film surface.Methylthiazol tetrazolium (MTT) assay demonstrated that PHBVHHx was better thanthe above four materials in promoting cell attachment and proliferation of fibroblastcell line L929 and osteoblast cell line MC3T3, respectively. Histological study usingrabbits proved that PHBVHHx was a fairly harmless implantable biomaterial. Thus,PHBVHHx with an adjustable mechanical property, combined with itsbiocompatibility, are in the process of developing into a new generation of bioimplantmaterial.更多还原