洋参生脉冻干粉针主要药效学作用及机制初探

【作者】 李欢；

【导师】 曹颖林； 周晓棉；

【作者基本信息】 沈阳药科大学 ， 药理学， 2007， 硕士

【摘要】 本文主要对洋参生脉冻干粉针的主要药效学作用及其可能作用机制进行了初步探讨，同时对该药的安全性（急性毒性）和一般药理学作用进行了研究。实验结果表明洋参生脉冻干粉针(32.0mg·kg^-1、16.0mg·kg^-1、8.0mg·kg^-1)可显著改善垂体后叶素诱发的大鼠急性心肌缺血心电图T波异常，明显对抗异丙肾上腺素诱导的急性心肌缺血大鼠心电图ST段的偏移；减少由异丙肾上腺素诱导的心肌坏死导致的心肌酶如天门冬氨基转移酶（AST）、乳酸脱氢酶（LDH）、肌酸磷酸激酶（CK）、肌酸激酶同工酶（CK-MB）的漏出，且高、中、低剂量作用(32.0mg·kg^-1、16.0mg·kg^-1、8.0mg·kg^-1)显著；高、中剂量(44.0mg·kg^-1、22.0mg·kg^-1)可显著抑制异丙肾上腺素诱导的小鼠心肌含水量和心肌指数的增加，能显著提高心肌、血清中SOD的活力，同时降低心肌、血清中MDA的含量。对麻醉大鼠血流动力学的考察表明，静脉注射洋参生脉冻干粉针使受试大鼠平均动脉压（MAP）、左心室收缩压（LVSP）、左室内压最大变化速率(±dp／dt_max)升高，左室舒张末期压（LVEDP）降低。以上结果表明洋参生脉冻干粉针在所选剂量范围内对缺血心肌有一定保护作用。实验结果表明洋参生脉冻干粉针高、中剂量(44.0mg·kg^-1、22.0mg·kg^-1)对氯仿诱发的小鼠心室纤颤的发生率有显著的抑制作用，高、中剂量(32.0mg·kg^-1、16.0mg·kg^-1)亦能显著缩短氯化钡诱发的大鼠心律失常的维持时间；洋参生脉冻干粉针(32.0mg·kg^-1、16.0mg·kg^-1)可延长小鼠气管夹闭后心电消失时间，各剂量(44.0mg·kg^-1、22.0mg·kg^-1、11.0mg·kg^-1)均能显著延长小鼠特异性缺氧条件下的存活时间。综合本文实验结果提示，洋参生脉冻干粉针具有一定的抗心肌缺血的作用，其机制可能与改善血流动力学各项指标，增加缺血心肌超氧化物歧化酶（SOD）活性等作用有关；对心律失常、心肌缺氧亦有保护作用，其作用机制有待进一步研究。更多还原

【Abstract】 The anti-myocardial ischemia effect and the mechanisms of YangShenShengMai freeze-dried power （YSSM） were preliminary studied. Toxicity and general pharmacology of it was studied too in this paper.YSSM (32.0mg.kg^-1, 16.0mg.kg_-1, 8.0mg.kg₊₁) could obviously improve the ischemic electrocardiogram T-wave induced by Pit. ST segment drifts on electrocardiogram in acute myocardial ischemia rats induced by Iso were obviously opposed by administration of YSSM (32.0mg.kg^-1, 16.0mg.kg^-1, 8.0mg.kg^-1). The releasement of AST, LDH, CK and CK-MB significantly increased in myocardial ischemia rats, YSSM (32.0mg.kg^-1、16.0mg.kg^-1, 8.0mg.kg^-1) treatment prevented these effects. YSSM (44.0mg.kg^-1、22.0mg.kg^-1)can significantly inhibit the increasing of MWC and MI induced by Iso. The activity of SOD in cardiac muscle and blood-serum can be significantly raised, the content of MDA in cardiac muscle and blood-serum can be significantly depressed all by YSSM (44.0mg.kg^-1、22.0mg.kg^-1). Through the hemodynamics study of anaesthetic rats, YSSM can significantly increased the MAP, LVSP and±dp/dt_max,, and depressed the LVEDE These results indicated that YSSM had the some protective effects on ischemia myocardium in the range of the dosage.YSSM (44.0mg’kg^-1, 22.0mg’kg^-1)can significantly inhibit the incidence rate of ventricular fibrillation induced by chloroform in mice, and shorten the maintenance time of arrhythmia induced by BaCl₂ in rats with the dosage 32mg.kg^-1 and 16mg.kg^-1. YSSM (44.0mg.kg^-1, 22.0mg.kg^-1)can extend the extinction time of ECG in the clamped trachea mice, and all the dosage (44.0mg.kg^-1、22.0mg.kg^-1、11.0mg.kg^-1)can significantly prolong the survival time in specificity hypoxia mice.Summarizing all the experimental results, YSSM showed some anti-myocardial ischemia effects. The mechanism maybe involved in ameliorating hemodynamical indexes, increasing the SOD activity. YSSM also have the effects on arrhythmia and myocardial anoxia, but there are more to do with the research of the mechanism.更多还原