【作者】 武晓宇；

【导师】 康广盛；

【作者基本信息】 黑龙江中医药大学 ， 方剂学， 2007， 硕士

【摘要】 目的：研究温胆汤对高脂血症大鼠血脂代谢的调节作用及其作用机理。方法：取雄性Wistar大鼠40只，体重(200±20)g，按随机数字表法进行完全随机分成正常对照组，模型对照组和温胆汤组，血脂康组及辛伐他汀组5组，每组8只。以脂肪乳剂灌饲法进行高脂血症的模型复制。在模型复制成功的基础上每日上午模型组，温胆汤组，血脂康组及辛伐他汀组给予脂肪乳剂10ml／kg，正常对照组给予同等剂量的蒸馏水，每日下午温胆汤组给予温胆汤6g／kg，血脂康组给予血脂康混悬液0.108g／kg，辛伐他汀组给予辛伐他汀混悬液0.9mg／kg，其余两组给予同等剂量的蒸馏水，连续灌胃30天，末次给药后禁食12小时摘取眼球取血，离心，进行生化测定TC、TG、HDL-C、LDL-C水平、MDA含量、SOD活力、LA、LPL、HL的活性。结果：温胆汤能显著降低实验性高脂血症大鼠血中的TC、TG水平，提高血清SOD活性，降低MDA含量，同时，温胆汤能提高肝脏总脂解酶(LA)和脂蛋白脂酶(LPL)活性，但对肝脂酶(HL)活性无明显影响。结论：温胆汤能有效调节机体脂质代谢，其机理可能是通过提高LA和LPL的活性而发挥其调脂作用，同时，温胆汤能有效降低机体内脂质过氧化程度，降低细胞受损程度，对动脉粥样硬化，冠心病的防治有一定的应用价值。更多还原

【Abstract】 Objectives: To study the effect and principle of Wendan decoction on blood-lipid of hyperlipidemia rats.Methods: Forty wister rats that weight about 180-220g are divided into five groups randomicity. control group, model control group, Wendan decoction group, Xuezhikang capsule group and Simvastatin group, each group has eight rats. In this study, animal model of hyperlipidemia in rats has been established by douched high fat emulsion; when the model has been succeed, every morning, Wendan decoction group, Xuezhikang capsule group and Simvastatin group were douched high fat emulsion 2.75g/kg, as well as control group were douched the same dose pure water; then the afternoon, Wendan decotion group were douched wendan decotion 6g/kg, Xuezhikang capsule group were given by lavage to Xuezhikang capsule by 0.108g/kg, Simvastatin group were given by lavage to Simvastatin tablet by 0.9mg/kg, others were given the same dose pure water for thirty days, Last dosage, they were abrosiaed for 12h, then gain blood by select the rats’ eyeball . Determin the concent of blood TC, TG , LDL-C, HDL-C, MOD and activity of SOD, LA, LPL and HL.Cunclusions: Wendan decoction has the effect on regulating blood-lipid metabolic disorder, the mechanism may be enhance the activity of LA and LPL as well as preventing lipid peroxidation and cells damaged, it shows Wendan decoction has an applied value on preventing and treating the happening and developing of AS and CHD diseases.Result: Wendan decoction has obviously reduced the concents of blood TC, TG and MDA in rats, it has obviously raisen the activity of SOD, at the same time, it has obviously raisen the activity of LA and LPL, but has no effect on HL.更多还原